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Int. J. Mol. Sci. 2014, 15(9), 15552-15570;

Structures and Functions of Qβ Replicase: Translation Factors beyond Protein Synthesis

National Institute of Advanced Industrial Sciences and Technology (AIST), Biomedical Research Institute, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
Received: 7 August 2014 / Revised: 27 August 2014 / Accepted: 29 August 2014 / Published: 2 September 2014
(This article belongs to the Special Issue Functions of Transfer RNAs)
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Qβ replicase is a unique RNA polymerase complex, comprising Qβ virus-encoded RNA-dependent RNA polymerase (the catalytic β-subunit) and three host-derived factors: translational elongation factor (EF) -Tu, EF-Ts and ribosomal protein S1. For almost fifty years, since the isolation of Qβ replicase, there have been several unsolved, important questions about the mechanism of RNA polymerization by Qβ replicase. Especially, the detailed functions of the host factors, EF-Tu, EF-Ts, and S1, in Qβ replicase, which are all essential in the Escherichia coli (E. coli) host for protein synthesis, had remained enigmatic, due to the absence of structural information about Qβ replicase. In the last five years, the crystal structures of the core Qβ replicase, consisting of the β-subunit, EF-Tu and Ts, and those of the core Qβ replicase representing RNA polymerization, have been reported. Recently, the structure of Qβ replicase comprising the β-subunit, EF-Tu, EF-Ts and the N-terminal half of S1, which is capable of initiating Qβ RNA replication, has also been reported. In this review, based on the structures of Qβ replicase, we describe our current understanding of the alternative functions of the host translational elongation factors and ribosomal protein S1 in Qβ replicase as replication factors, beyond their established functions in protein synthesis. View Full-Text
Keywords: Qβ replicase; RNA-dependent RNA polymerase; EF-Tu; EF-Ts; ribosomal protein S1 Qβ replicase; RNA-dependent RNA polymerase; EF-Tu; EF-Ts; ribosomal protein S1

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Tomita, K. Structures and Functions of Qβ Replicase: Translation Factors beyond Protein Synthesis. Int. J. Mol. Sci. 2014, 15, 15552-15570.

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