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Int. J. Mol. Sci. 2014, 15(9), 15287-15303; doi:10.3390/ijms150915287

Dual Targeting Biomimetic Liposomes for Paclitaxel/DNA Combination Cancer Treatment

1
Department of Clinical Lab, Jinan Stomatological Hospital, Jinan 250001, China
2
Department of Pharmacy, Shandong Provincial Qian Foshan Hospital, Jinan 250014, China
*
Author to whom correspondence should be addressed.
Received: 7 May 2014 / Revised: 15 August 2014 / Accepted: 21 August 2014 / Published: 29 August 2014
(This article belongs to the Special Issue Biomimetic and Functional Materials)
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Abstract

Combinations of chemotherapeutic drugs with nucleic acid has shown great promise in cancer therapy. In the present study, paclitaxel (PTX) and DNA were co-loaded in the hyaluronic acid (HA) and folate (FA)-modified liposomes (HA/FA/PPD), to obtain the dual targeting biomimetic nanovector. The prepared HA/FA/PPD exhibited nanosized structure and narrow size distributions (247.4 ± 4.2 nm) with appropriate negative charge of −25.40 ± 2.7 mV. HA/FA/PD (PTX free HA/FA/PPD) showed almost no toxicity on murine malignant melanoma cell line (B16) and human hepatocellular carcinoma cell line (HepG2) (higher than 80% cell viability), demonstrating the safety of the blank nanovector. In comparison with the FA-modified PTX/DNA co-loaded liposomes (FA/PPD), HA/FA/PPD showed significant superiority in protecting the nanoparticles from aggregation in the presence of plasma and degradation by DNase I. Moreover, HA/FA/PPD could also significantly improve the transfection efficiency and cellular internalization rates on B16 cells comparing to that of FA/PPD (p < 0.05) and PPD (p < 0.01), demonstrating the great advantages of dual targeting properties. Furthermore, fluorescence microscope and flow cytometry results showed that PTX and DNA could be effectively co-delivered into the same tumor cell via HA/FA/PPD, contributing to PTX/DNA combination cancer treatment. In conclusion, the obtained HA/FA/PPD in the study could effectively target tumor cells, enhance transfection efficiency and subsequently achieve the co-delivery of PTX and DNA, displaying great potential for optimal combination therapy. View Full-Text
Keywords: hyaluronic acid; folate; biomimetic liposomes; paclitaxel; DNA; co-delivery hyaluronic acid; folate; biomimetic liposomes; paclitaxel; DNA; co-delivery
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MDPI and ACS Style

Liu, G.-X.; Fang, G.-Q.; Xu, W. Dual Targeting Biomimetic Liposomes for Paclitaxel/DNA Combination Cancer Treatment. Int. J. Mol. Sci. 2014, 15, 15287-15303.

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