Next Article in Journal
Dual Targeting Biomimetic Liposomes for Paclitaxel/DNA Combination Cancer Treatment
Previous Article in Journal
The Adenosine Deaminase Gene Polymorphism Is Associated with Chronic Heart Failure Risk in Chinese
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(9), 15272-15286; doi:10.3390/ijms150915272

Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum

1
Division of Applied Bioscience & Biotechnology, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Korea
2
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Korea
3
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration (RDA), Eumseung 369-873, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 6 June 2014 / Revised: 29 July 2014 / Accepted: 11 August 2014 / Published: 28 August 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3082 KB, uploaded 28 August 2014]   |  

Abstract

Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2013 to identify the new or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected for molecular identification with the internal transcribed spacer (ITS) gene. Phylogenetic analysis revealed that all isolates belonged to Ascomycota including the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and Nemania. Two Colletotrichum species were identified at the species level, using three genes including internal transcribed spacer (ITS), glycerol-3-phosphate dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along with morphological observations. The fungal isolates, CNU122031 and CNU122032 were identified as Colletotrichum fructicola and C. brevisporum, respectively. Morphological observations also well supported the molecular identification. C. brevisporum is represented unrecorded species in Korea and C. fructicola is the first record from the host plant. View Full-Text
Keywords: colletotrichum brevisporum; colletotrichum fructicola; endophytic fungi; lycium chinense mill; new records colletotrichum brevisporum; colletotrichum fructicola; endophytic fungi; lycium chinense mill; new records
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Paul, N.C.; Lee, H.B.; Lee, J.H.; Shin, K.S.; Ryu, T.H.; Kwon, H.R.; Kim, Y.K.; Youn, Y.N.; Yu, S.H. Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum. Int. J. Mol. Sci. 2014, 15, 15272-15286.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top