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Int. J. Mol. Sci. 2014, 15(9), 15188-15209; doi:10.3390/ijms150915188

Compositional and Proteomic Analyses of Genetically Modified Broccoli (Brassica oleracea var. italica) Harboring an Agrobacterial Gene

1
Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan
2
Department of Anatomy, School of Medicine, China Medical University, Taichung 40402, Taiwan
3
Unit, Taiwan Agricultural Research Institute, Wufeng, Taichung 41362, Taiwan
4
Department of Bioindustry Technology, Dayeh University No. 168, University Rd., Dacun, Changhua 51591, Taiwan
*
Author to whom correspondence should be addressed.
Received: 17 June 2014 / Revised: 19 August 2014 / Accepted: 25 August 2014 / Published: 28 August 2014
(This article belongs to the Special Issue Detection and Safety Assessment of Genetically Modified Organisms)
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Abstract

Previously, we showed improved shelf life for agrobacterial isopentenyltransferase (ipt) transgenic broccoli (Brassica oleracea var. italica), with yield comparable to commercial varieties, because of the protection mechanism offered by molecular chaperones and stress-related proteins. Here, we used proximate analysis to examine macronutrients, chemical and mineral constituents as well as anti-nutrient and protein changes of ipt-transgenic broccoli and corresponding controls. We also preliminarily assessed safety in mice. Most aspects were comparable between ipt-transgenic broccoli and controls, except for a significant increase in carbohydrate level and a decrease in magnesium content in ipt-transgenic lines 101, 102 and 103, as compared with non-transgenic controls. In addition, the anti-nutrient glucosinolate content was increased and crude fat content decreased in inbred control 104 and transgenic lines as compared with the parental control, “Green King”. Gel-based proteomics detected more than 50 protein spots specifically found in ipt-transgenic broccoli at harvest and after cooking; one-third of these proteins showed homology to potential allergens that also play an important role in plant defense against stresses and senescence. Mice fed levels of ipt-transgenic broccoli mimicking the 120 g/day of broccoli eaten by a 60-kg human adult showed normal growth and immune function. In conclusion, the compositional and proteomic changes attributed to the transgenic ipt gene did not affect the growth and immune response of mice under the feeding regimes examined. View Full-Text
Keywords: allergen; Brassica oleracea var. italica; broccoli; proteomics; glucosinolate; isopentenyltransferase (ipt) allergen; Brassica oleracea var. italica; broccoli; proteomics; glucosinolate; isopentenyltransferase (ipt)
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MDPI and ACS Style

Liu, M.-S.; Ko, M.-H.; Li, H.-C.; Tsai, S.-J.; Lai, Y.-M.; Chang, Y.-M.; Wu, M.-T.; Chen, L.-F.O. Compositional and Proteomic Analyses of Genetically Modified Broccoli (Brassica oleracea var. italica) Harboring an Agrobacterial Gene. Int. J. Mol. Sci. 2014, 15, 15188-15209.

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