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Int. J. Mol. Sci. 2014, 15(8), 14835-14847; doi:10.3390/ijms150814835

Influence of Gradual Elongation to the Patella Tendon Insertion in Rabbits

1
Department of Orthopaedic Surgery, Ibaraki Prefectural University of Health Sciences, 4669-2 Ami Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
2
Department of Agriculture, Ibaraki University, 3-21-1 Chuuou, Ami-machi, Inashiki-gun, Ibaraki 300-0393, Japan
3
Department of Rehabilitation Medicine, Ibaraki Prefectural University of Health Sciences, 4669-2 Ami Ami-machi, Inashiki-gun, Ibaraki 300-0394, Japan
4
Department of Orthopaedic Surgery, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan
*
Author to whom correspondence should be addressed.
Received: 21 May 2014 / Revised: 13 August 2014 / Accepted: 18 August 2014 / Published: 22 August 2014
(This article belongs to the Special Issue The Chondrocyte Phenotype in Cartilage Biology)
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Abstract

The purpose of this study was to examine the histological changes at the patella tendon (PT) insertion site under gradual elongation in rabbits. Gradual elongation of the PT was performed using external fixation for 4 weeks, with a lengthening speed of 0.5 mm/day (elongation group; n = 24). Rabbits in the sham group underwent the same surgical procedure without gradual elongation (sham group; n = 24). Eight animals were sacrificed 1, 2 and 4 weeks after surgery in each group, respectively. Average thicknesses of stained glycosaminoglycan (GAGs) areas by Safranin-O staining in the total cartilage layer and the uncalcified fibrocartilage layer in the elongation group were significantly higher than that in the sham group at 4 weeks (p < 0.05) and that in the intact PT group (n = 6, p < 0.05). In the elongation group, the peak in the average thicknesses of the stained GAGs areas in the total cartilage layer and the uncalcified fibrocartilage layer were observed at 4 weeks. Gradual elongation of PT insertion significantly affected the increase in the average thicknesses of the stained GAGs areas in the cartilage layer especially in the uncalcified fibrocartilage layer at 4 weeks in rabbits. Clinically, insertions of tendon and ligament can extend during gradual elongation using external fixation more than 4 weeks after the operation. View Full-Text
Keywords: insertion; chondrocytes apoptosis; chondrocyte proliferation; glycosaminoglycan; gradual elongation; external fixation insertion; chondrocytes apoptosis; chondrocyte proliferation; glycosaminoglycan; gradual elongation; external fixation
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Mutsuzaki, H.; Nakajima, H.; Wadano, Y.; Watanabe, S.; Sakane, M. Influence of Gradual Elongation to the Patella Tendon Insertion in Rabbits. Int. J. Mol. Sci. 2014, 15, 14835-14847.

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