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Int. J. Mol. Sci. 2014, 15(8), 14442-14455; doi:10.3390/ijms150814442

Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

1
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2
Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing 210095, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 July 2014 / Revised: 30 July 2014 / Accepted: 12 August 2014 / Published: 19 August 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior. View Full-Text
Keywords: Chrysanthemum morifolium; phylogenetic analysis; stress response; transcription pattern Chrysanthemum morifolium; phylogenetic analysis; stress response; transcription pattern
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Song, A.; Li, P.; Jiang, J.; Chen, S.; Li, H.; Zeng, J.; Shao, Y.; Zhu, L.; Zhang, Z.; Chen, F. Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors. Int. J. Mol. Sci. 2014, 15, 14442-14455.

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