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Int. J. Mol. Sci. 2014, 15(8), 13697-13719; doi:10.3390/ijms150813697
Article

A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting

1,* , 2
, 3
, 4
, 1
 and 5,6
1 Department of Laboratory Medicine, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, Italy 2 Gene Expression-Microarrays Laboratory, Bambino Gesù Children's Hospital, IRCCS,Piazza Sant'Onofrio 4, Rome 00165, Italy 3 Neonatal Intensive Care Unit, Department of Medical and Surgical Neonatology,Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4, Rome 00165, Italy 4 Department of Laboratory Medicine, San Camillo Hospital, Viale Vittorio Veneto 18,Treviso 31100, Italy 5 Parasitology Unit, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4,Rome 00165, Italy 6 Metagenomics Unit, Bambino Gesù Children's Hospital, IRCCS, Piazza Sant'Onofrio 4,Rome 00165, Italy
* Author to whom correspondence should be addressed.
Received: 23 April 2014 / Revised: 25 June 2014 / Accepted: 18 July 2014 / Published: 8 August 2014
(This article belongs to the Special Issue Advances in Proteomic Research)
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Abstract

She-donkey’s milk (DM) and goat’s milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.
Keywords: milk; adulteration; MALDI-TOF MS milk; adulteration; MALDI-TOF MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Di Girolamo, F.; Masotti, A.; Salvatori, G.; Scapaticci, M.; Muraca, M.; Putignani, L. A Sensitive and Effective Proteomic Approach to Identify She-Donkey’s and Goat’s Milk Adulterations by MALDI-TOF MS Fingerprinting. Int. J. Mol. Sci. 2014, 15, 13697-13719.

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