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Int. J. Mol. Sci. 2014, 15(8), 13596-13614; doi:10.3390/ijms150813596

Identification of Cold-Responsive miRNAs and Their Target Genes in Nitrogen-Fixing Nodules of Soybean

1
The State Key Laboratory of Plant Cell & Chromosome Engineering, Center of Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei 050021, China
2
College of Life Sciences, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 29 May 2014 / Revised: 14 July 2014 / Accepted: 17 July 2014 / Published: 5 August 2014
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Abstract

As a warm climate species, soybean is highly sensitive to chilling temperatures. Exposure to chilling temperatures causes a significant reduction in the nitrogen fixation rate in soybean plants and subsequent yield loss. However, the molecular basis for the sensitivity of soybean to chilling is poorly understood. In this study, we identified cold-responsive miRNAs in nitrogen-fixing nodules of soybean. Upon chilling, the expression of gma-miR397a, gma-miR166u and gma-miR171p was greatly upregulated, whereas the expression of gma-miR169c, gma-miR159b, gma-miR319a/b and gma-miR5559 was significantly decreased. The target genes of these miRNAs were predicted and validated using 5' complementary DNA ends (5'-RACE) experiments, and qPCR analysis identified putative genes targeted by the cold-responsive miRNAs in response to chilling temperatures. Taken together, our results reveal that miRNAs may be involved in the protective mechanism against chilling injury in mature nodules of soybean. View Full-Text
Keywords: microRNAs; functional nodules; low temperature; Glycine max (L.) Merrill microRNAs; functional nodules; low temperature; Glycine max (L.) Merrill
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhang, S.; Wang, Y.; Li, K.; Zou, Y.; Chen, L.; Li, X. Identification of Cold-Responsive miRNAs and Their Target Genes in Nitrogen-Fixing Nodules of Soybean. Int. J. Mol. Sci. 2014, 15, 13596-13614.

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