Next Article in Journal
Overexpression of Shox2 Leads to Congenital Dysplasia of the Temporomandibular Joint in Mice
Next Article in Special Issue
Synthesis and Properties of Poly(l-lactide)-b-poly (l-phenylalanine) Hybrid Copolymers
Previous Article in Journal
DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide
Previous Article in Special Issue
Cellulose Nanocrystals/ZnO as a Bifunctional Reinforcing Nanocomposite for Poly(vinyl alcohol)/Chitosan Blend Films: Fabrication, Characterization and Properties
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(7), 13123-13134; doi:10.3390/ijms150713123

Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin

1
Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
2
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan
*
Author to whom correspondence should be addressed.
Received: 1 May 2014 / Revised: 14 July 2014 / Accepted: 15 July 2014 / Published: 23 July 2014
(This article belongs to the Special Issue Biodegradable Materials)
View Full-Text   |   Download PDF [1315 KB, uploaded 23 July 2014]   |  

Abstract

Cholesteryl group-modified tilapia gelatins (Chol-T-Gltns) with various Chol contents from 3 to 69 mol % per amino group of Gltn were prepared for the assembly of liposomes and cells. Liposomes were physically crosslinked by anchoring Chol groups of Chol-T-Gltns into lipid membranes. The resulting liposome gels were enzymatically degraded by addition of collagenase. Liposome gels prepared using Chol-T-Gltn with high Chol content (69Chol-T-Gltn) showed slower enzymatic degradation when compared with gels prepared using Chol-T-Gltn with low Chol content (3Chol-T-Gltn). The hepatocyte cell line HepG2 showed good assembly properties and no cytotoxic effects after addition of 69Chol-T-Gltns. In addition, the number of HepG2 cells increased with concentration of 69Chol-T-Gltns. Therefore, Chol-T-Gltn, particularly, 69Chol-T-Gltn, can be used as an assembling material for liposomes and various cell types. The resulting organization can be applied to various biomedical fields, such as drug delivery systems, tissue engineering and regenerative medicine. View Full-Text
Keywords: hydrophobically modified gelatin; cholesterol; assembly; liposome; cell hydrophobically modified gelatin; cholesterol; assembly; liposome; cell
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Taguchi, T.; Endo, Y. Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin. Int. J. Mol. Sci. 2014, 15, 13123-13134.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top