Abstract: Genomic DNA is under constant assault by endogenous and exogenous DNA damaging agents. DNA breakage can represent a major threat to genome integrity but can also be necessary for genome function. Here we present approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) at the genome-wide scale by two methods called DSB- and SSB-Seq, respectively. We tested these methods in human colon cancer cells and validated the results using the Topoisomerase II (Top2)-poisoning agent etoposide (ETO). Our results show that the combination of ETO treatment with break-mapping techniques is a powerful method to elaborate the pattern of Top2 enzymatic activity across the genome.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Baranello, L.; Kouzine, F.; Wojtowicz, D.; Cui, K.; Przytycka, T.M.; Zhao, K.; Levens, D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. Int. J. Mol. Sci. 2014, 15, 13111-13122.
Baranello L, Kouzine F, Wojtowicz D, Cui K, Przytycka TM, Zhao K, Levens D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. International Journal of Molecular Sciences. 2014; 15(7):13111-13122.
Baranello, Laura; Kouzine, Fedor; Wojtowicz, Damian; Cui, Kairong; Przytycka, Teresa M.; Zhao, Keji; Levens, David. 2014. "DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide." Int. J. Mol. Sci. 15, no. 7: 13111-13122.