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Int. J. Mol. Sci. 2014, 15(7), 13111-13122; doi:10.3390/ijms150713111

DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide

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Received: 31 May 2014; in revised form: 9 July 2014 / Accepted: 14 July 2014 / Published: 23 July 2014
(This article belongs to the Special Issue Identification and Roles of the Structure of DNA)
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Abstract: Genomic DNA is under constant assault by endogenous and exogenous DNA damaging agents. DNA breakage can represent a major threat to genome integrity but can also be necessary for genome function. Here we present approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) at the genome-wide scale by two methods called DSB- and SSB-Seq, respectively. We tested these methods in human colon cancer cells and validated the results using the Topoisomerase II (Top2)-poisoning agent etoposide (ETO). Our results show that the combination of ETO treatment with break-mapping techniques is a powerful method to elaborate the pattern of Top2 enzymatic activity across the genome.
Keywords: topoisomerases; DNA damage; transcription topoisomerases; DNA damage; transcription
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Baranello, L.; Kouzine, F.; Wojtowicz, D.; Cui, K.; Przytycka, T.M.; Zhao, K.; Levens, D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. Int. J. Mol. Sci. 2014, 15, 13111-13122.

AMA Style

Baranello L, Kouzine F, Wojtowicz D, Cui K, Przytycka TM, Zhao K, Levens D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. International Journal of Molecular Sciences. 2014; 15(7):13111-13122.

Chicago/Turabian Style

Baranello, Laura; Kouzine, Fedor; Wojtowicz, Damian; Cui, Kairong; Przytycka, Teresa M.; Zhao, Keji; Levens, David. 2014. "DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide." Int. J. Mol. Sci. 15, no. 7: 13111-13122.

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