DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide
AbstractGenomic DNA is under constant assault by endogenous and exogenous DNA damaging agents. DNA breakage can represent a major threat to genome integrity but can also be necessary for genome function. Here we present approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) at the genome-wide scale by two methods called DSB- and SSB-Seq, respectively. We tested these methods in human colon cancer cells and validated the results using the Topoisomerase II (Top2)-poisoning agent etoposide (ETO). Our results show that the combination of ETO treatment with break-mapping techniques is a powerful method to elaborate the pattern of Top2 enzymatic activity across the genome.
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Baranello, L.; Kouzine, F.; Wojtowicz, D.; Cui, K.; Przytycka, T.M.; Zhao, K.; Levens, D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. Int. J. Mol. Sci. 2014, 15, 13111-13122.
Baranello L, Kouzine F, Wojtowicz D, Cui K, Przytycka TM, Zhao K, Levens D. DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide. International Journal of Molecular Sciences. 2014; 15(7):13111-13122.Chicago/Turabian Style
Baranello, Laura; Kouzine, Fedor; Wojtowicz, Damian; Cui, Kairong; Przytycka, Teresa M.; Zhao, Keji; Levens, David. 2014. "DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide." Int. J. Mol. Sci. 15, no. 7: 13111-13122.