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Int. J. Mol. Sci. 2014, 15(7), 11847-11861; doi:10.3390/ijms150711847

Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

1
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
2
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China
3
College of Life Sciences, China Jiliang University, Hangzhou 310018, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 15 May 2014 / Revised: 24 June 2014 / Accepted: 24 June 2014 / Published: 3 July 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Oryza meyeriana is highly resistant to rice bacterial blight (BB) and this resistance trait has been transferred to cultivated rice (O. sativa) using asymmetric somatic hybridization. However, no resistance genes have yet been cloned. In the present study, a progeny of the somatic hybridization with high BB resistance was crossed with a rice cultivar with high BB susceptibility to develop an F2 population. Using bulked segregant analysis (BSA), 17 polymorphic markers that were linked to rice BB resistance were obtained through scanning a total of 186 simple sequence repeats (SSR) and sequence-tagged site (STS) markers, evenly distributed on 12 chromosomes. A genetic linkage map was then constructed based on the 17 linkage markers and the F2 segregating population, which was followed by mapping for quantitative trait loci (QTLs) for BB resistance. Three QTLs were identified on chromosomes 1, 3 and 5, respectively, and the alleles of the resistant parent at any of the QTLs increased BB resistance. All of the three QTLs had a strong effect on resistance, explaining about 21.5%, 12.3% and 39.2% of the resistance variance, respectively. These QTLs were different from the loci of the BB resistance genes that have been identified in previous studies. The QTLs mapped in this work will facilitate the isolation of novel BB resistance genes and their utilization in rice resistance breeding. View Full-Text
Keywords: quantitative trait loci; mapping; bacterial blight resistance; bulked segregant analysis; Oryza meyeriana quantitative trait loci; mapping; bacterial blight resistance; bulked segregant analysis; Oryza meyeriana
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Han, X.; Yang, Y.; Wang, X.; Zhou, J.; Zhang, W.; Yu, C.; Cheng, C.; Cheng, Y.; Yan, C.; Chen, J. Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis. Int. J. Mol. Sci. 2014, 15, 11847-11861.

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