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Int. J. Mol. Sci. 2014, 15(7), 11523-11538; doi:10.3390/ijms150711523
Article

Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors

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Received: 20 March 2014; in revised form: 11 April 2014 / Accepted: 29 April 2014 / Published: 27 June 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
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Abstract: Eukaryotic translation initiation factors are the principal molecular effectors regulating the process converting nucleic acid to functional protein. Commonly referred to as eIFs (eukaryotic initiation factors), this suite of proteins is comprised of at least 25 individual subunits that function in a coordinated, regulated, manner during mRNA translation. Multiple facets of eIF regulation have yet to be elucidated; however, many of the necessary protein factors are phosphorylated. Herein, we have isolated, identified and quantified phosphosites from eIF2, eIF3, and eIF4G generated from log phase grown HeLa cell lysates. Our investigation is the first study to globally quantify eIF phosphosites and illustrates differences in abundance of phosphorylation between the residues of each factor. Thus, identification of those phosphosites that exhibit either high or low levels of phosphorylation under log phase growing conditions may aid researchers to concentrate their investigative efforts to specific phosphosites that potentially harbor important regulatory mechanisms germane to mRNA translation.
Keywords: mass spectrometry; eukaryotic initiation factor; translation; phosphorylation quantification mass spectrometry; eukaryotic initiation factor; translation; phosphorylation quantification
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Andaya, A.; Villa, N.; Jia, W.; Fraser, C.S.; Leary, J.A. Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors. Int. J. Mol. Sci. 2014, 15, 11523-11538.

AMA Style

Andaya A, Villa N, Jia W, Fraser CS, Leary JA. Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors. International Journal of Molecular Sciences. 2014; 15(7):11523-11538.

Chicago/Turabian Style

Andaya, Armann; Villa, Nancy; Jia, Weitao; Fraser, Christopher S.; Leary, Julie A. 2014. "Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors." Int. J. Mol. Sci. 15, no. 7: 11523-11538.



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