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Int. J. Mol. Sci. 2014, 15(7), 11403-11415; doi:10.3390/ijms150711403

Genetic Variants of APOC3 Promoter and HLA-B Genes in an HIV Infected Cohort in Northern South Africa: A Pilot Study

1
HIV/AIDS & Global Health Research Programme, Department of Microbiology, University of Venda, Thohoyandou 0950, South Africa
2
Department of Microbiology and Parasitology, Faculty of Science, University of Buea, Buea Box 63, Cameroon
3
Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa
4
Department of Biochemistry, University of Pretoria, Pretoria 0002, South Africa
*
Author to whom correspondence should be addressed.
Received: 28 December 2013 / Revised: 17 March 2014 / Accepted: 11 April 2014 / Published: 26 June 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Metabolic disorders and hypersensitivities affect tolerability and impact adherence to highly active antiretroviral therapy (HAART). The aim of this study was to determine the prevalence of C-482T/T-455C variants in the Apolipoprotein C3 (APOC3) promoter gene and Human leukocyte antigen (HLA)-B*57:01, known to impact lipid metabolic disorders and hypersensitivity respectively; and to correlate genotypes with gender, CD4+ cell count and viral load in an HIV infected cohort in northern South Africa. Frequencies of C-482 and T-455 polymorphisms in APOC3 were determined by restriction fragment length polymorphism analysis. Allele determination for HLA-B was performed with Assign SBT software in an HLA library. Analysis of APOC3 C-482 site revealed a prevalence of 196/199 (98.5%) for CC, 1/199 (0.5%) for CT and 2/199 (1.0%) for TT genotype (p = 0.000 with 1° of freedom; χ2 = 126.551). For the T-455 site, prevalences were: 69/199 (35%) for TT and 130/199 (65%) for the CC genotype (p = 0.000 with 1° of freedom; χ2 = 199). There was no association between gender and the presence of −482 (p = 1; χ2 = 0.00001) or −455 genotypes (p = 0.1628; χ2 = 1.9842). There was no significant difference in the increase in CD4+ cell count irrespective of genotypes. Significant increases in CD4+ cell count were observed in males and females considering the −455C genotype, but not in males for the −455T genotype. Viral load decreases were significant with the −455C and −482C genotypes irrespective of gender. HLA-B*57:01 was not identified in the study cohort. The apparently high prevalence of APOC3 T-455CC genotype needs confirmation with a larger samples size and triglyceride measurements to support screening of patients to pre-empt HAART associated lipid disorders. View Full-Text
Keywords: genetic variants; APOC3; HLA-B; highly active antiretroviral therapy; lipid disorders; South Africa genetic variants; APOC3; HLA-B; highly active antiretroviral therapy; lipid disorders; South Africa
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Masebe, T.; Bessong, P.O.; Ndip, R.N.; Meyer, D. Genetic Variants of APOC3 Promoter and HLA-B Genes in an HIV Infected Cohort in Northern South Africa: A Pilot Study. Int. J. Mol. Sci. 2014, 15, 11403-11415.

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