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Int. J. Mol. Sci. 2014, 15(6), 9644-9669; doi:10.3390/ijms15069644
Article

Flowering as the Most Highly Sensitive Period of Grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria Dieback Agents Neofusicoccum parvum and Diplodia seriata Infection

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Received: 8 April 2014; in revised form: 4 May 2014 / Accepted: 13 May 2014 / Published: 30 May 2014
(This article belongs to the collection Advances in Proteomic Research)
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Abstract: Botryosphaeria dieback is a fungal grapevine trunk disease that currently represents a threat for viticulture worldwide because of the important economical losses due to reduced yield of affected plants and their premature death. Neofusicoccum parvum and Diplodia seriata are among the causal agents. Vine green stems were artificially infected with N. parvum or D. seriata at the onset of three different phenological stages (G stage (separated clusters), flowering and veraison). Highest mean lesion lengths were recorded at flowering. Major proteome changes associated to artificial infections during the three different phenological stages were also reported using two dimensional gel electrophoresis (2D)-based analysis. Twenty (G stage), 15 (flowering) and 13 (veraison) differentially expressed protein spots were subjected to nanoLC-MS/MS and a total of 247, 54 and 25 proteins were respectively identified. At flowering, a weaker response to the infection was likely activated as compared to the other stages, and some defense-related proteins were even down regulated (e.g., superoxide dismutase, major latex-like protein, and pathogenesis related protein 10). Globally, the flowering period seemed to represent the period of highest sensitivity of grapevine to Botryosphaeria dieback agent infection, possibly being related to the high metabolic activity in the inflorescences.
Keywords: Botryosphaeria dieback; Neofusicoccum parvum; Diplodia seriata; plant proteomics; two dimensional gel electrophoresis; defense-related proteins Botryosphaeria dieback; Neofusicoccum parvum; Diplodia seriata; plant proteomics; two dimensional gel electrophoresis; defense-related proteins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Spagnolo, A.; Larignon, P.; Magnin-Robert, M.; Hovasse, A.; Cilindre, C.; Van Dorsselaer, A.; Clément, C.; Schaeffer-Reiss, C.; Fontaine, F. Flowering as the Most Highly Sensitive Period of Grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria Dieback Agents Neofusicoccum parvum and Diplodia seriata Infection. Int. J. Mol. Sci. 2014, 15, 9644-9669.

AMA Style

Spagnolo A, Larignon P, Magnin-Robert M, Hovasse A, Cilindre C, Van Dorsselaer A, Clément C, Schaeffer-Reiss C, Fontaine F. Flowering as the Most Highly Sensitive Period of Grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria Dieback Agents Neofusicoccum parvum and Diplodia seriata Infection. International Journal of Molecular Sciences. 2014; 15(6):9644-9669.

Chicago/Turabian Style

Spagnolo, Alessandro; Larignon, Philippe; Magnin-Robert, Maryline; Hovasse, Agnès; Cilindre, Clara; Van Dorsselaer, Alain; Clément, Christophe; Schaeffer-Reiss, Christine; Fontaine, Florence. 2014. "Flowering as the Most Highly Sensitive Period of Grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria Dieback Agents Neofusicoccum parvum and Diplodia seriata Infection." Int. J. Mol. Sci. 15, no. 6: 9644-9669.


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