Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding
AbstractSpecific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. View Full-Text
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Waseem, S.; Allen, M.A.; Schreier, S.; Udomsangpetch, R.; Bhakdi, S.C. Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding. Int. J. Mol. Sci. 2014, 15, 8821-8834.
Waseem S, Allen MA, Schreier S, Udomsangpetch R, Bhakdi SC. Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding. International Journal of Molecular Sciences. 2014; 15(5):8821-8834.Chicago/Turabian Style
Waseem, Shahid; Allen, Michael A.; Schreier, Stefan; Udomsangpetch, Rachanee; Bhakdi, Sebastian C. 2014. "Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding." Int. J. Mol. Sci. 15, no. 5: 8821-8834.