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Int. J. Mol. Sci. 2014, 15(5), 8821-8834; doi:10.3390/ijms15058821

Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding

1
Department of Pathobiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
2
Department of Physics, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
3
Institute for Innovative Learning, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakorn Pathom 73170, Thailand
4
X-Zell Biotec Co., Ltd., Na-Nakorn Building, 99/349, Moo 2, Chaengwattana Road, Laksi, Bangkok 10210, Thailand
*
Author to whom correspondence should be addressed.
Received: 3 February 2014 / Revised: 23 April 2014 / Accepted: 24 April 2014 / Published: 19 May 2014
(This article belongs to the Section Biomaterial Sciences)
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Abstract

Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 − exp(−kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding. View Full-Text
Keywords: high gradient magnetic separation; bead-cell binding; iron content; paramagnetic nanoparticles; magnetic labeling; cell separation high gradient magnetic separation; bead-cell binding; iron content; paramagnetic nanoparticles; magnetic labeling; cell separation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Waseem, S.; Allen, M.A.; Schreier, S.; Udomsangpetch, R.; Bhakdi, S.C. Antibody-Conjugated Paramagnetic Nanobeads: Kinetics of Bead-Cell Binding. Int. J. Mol. Sci. 2014, 15, 8821-8834.

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