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Int. J. Mol. Sci. 2014, 15(4), 6609-6624; doi:10.3390/ijms15046609
Article

Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients

1,* , 1
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, 3,5
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1 Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland 2 Polish-Japanese Institute of Information Technologies, Koszykowa 86, 02-008 Warszawa, Poland 3 Institute of Mathematics and Computer Science, Wroclaw University of Technology, Janiszewskiego 14a, 50-370 Wroclaw, Poland 4 Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland 5 MedicWave AB, Kristian IV:s vag 3, 302 50 Halmstad, Sweden 6 Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland 7 Biomedical Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK
* Author to whom correspondence should be addressed.
Received: 30 January 2014 / Revised: 6 March 2014 / Accepted: 3 April 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
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Abstract

Cancer radiotherapy (RT) induces response of the whole patient’s body that could be detected at the blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses and volumes of irradiated tissue. Sixty-six patients treated with conformal RT because of head and neck cancer were enrolled in the study. Blood samples were collected before, during and about one month after the end of RT. Lipid extracts were analyzed using MALDI-oa-ToF mass spectrometry in positive ionization mode. The major changes were observed when pre-treatment and within-treatment samples were compared. Levels of several identified phosphatidylcholines, including (PC34), (PC36) and (PC38) variants, and lysophosphatidylcholines, including (LPC16) and (LPC18) variants, were first significantly decreased and then increased in post-treatment samples. Intensities of changes were correlated with doses of radiation received by patients. Of note, such correlations were more frequent when low-to-medium doses of radiation delivered during conformal RT to large volumes of normal tissues were analyzed. Additionally, some radiation-induced changes in serum lipidome were associated with toxicity of the treatment. Obtained results indicated the involvement of choline-related signaling and potential biological importance of exposure to clinically low/medium doses of radiation in patient’s body response to radiation.
Keywords: dose-volume effect; intensity-modulated radiation therapy; mass spectrometry; radiation toxicity; serum lipidome dose-volume effect; intensity-modulated radiation therapy; mass spectrometry; radiation toxicity; serum lipidome
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Jelonek, K.; Pietrowska, M.; Ros, M.; Zagdanski, A.; Suchwalko, A.; Polanska, J.; Marczyk, M.; Rutkowski, T.; Skladowski, K.; Clench, M.R.; Widlak, P. Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients. Int. J. Mol. Sci. 2014, 15, 6609-6624.

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