Int. J. Mol. Sci. 2014, 15(4), 6609-6624; doi:10.3390/ijms15046609
Article

Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients

1,* email, 1email, 1,2email, 3,5email, 4,5email, 6email, 6email, 1email, 1email, 7email and 1email
Received: 30 January 2014; in revised form: 6 March 2014 / Accepted: 3 April 2014 / Published: 17 April 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Cancer radiotherapy (RT) induces response of the whole patient’s body that could be detected at the blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses and volumes of irradiated tissue. Sixty-six patients treated with conformal RT because of head and neck cancer were enrolled in the study. Blood samples were collected before, during and about one month after the end of RT. Lipid extracts were analyzed using MALDI-oa-ToF mass spectrometry in positive ionization mode. The major changes were observed when pre-treatment and within-treatment samples were compared. Levels of several identified phosphatidylcholines, including (PC34), (PC36) and (PC38) variants, and lysophosphatidylcholines, including (LPC16) and (LPC18) variants, were first significantly decreased and then increased in post-treatment samples. Intensities of changes were correlated with doses of radiation received by patients. Of note, such correlations were more frequent when low-to-medium doses of radiation delivered during conformal RT to large volumes of normal tissues were analyzed. Additionally, some radiation-induced changes in serum lipidome were associated with toxicity of the treatment. Obtained results indicated the involvement of choline-related signaling and potential biological importance of exposure to clinically low/medium doses of radiation in patient’s body response to radiation.
Keywords: dose-volume effect; intensity-modulated radiation therapy; mass spectrometry; radiation toxicity; serum lipidome
PDF Full-text Download PDF Full-Text [927 KB, uploaded 19 June 2014 05:36 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Jelonek, K.; Pietrowska, M.; Ros, M.; Zagdanski, A.; Suchwalko, A.; Polanska, J.; Marczyk, M.; Rutkowski, T.; Skladowski, K.; Clench, M.R.; Widlak, P. Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients. Int. J. Mol. Sci. 2014, 15, 6609-6624.

AMA Style

Jelonek K, Pietrowska M, Ros M, Zagdanski A, Suchwalko A, Polanska J, Marczyk M, Rutkowski T, Skladowski K, Clench MR, Widlak P. Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients. International Journal of Molecular Sciences. 2014; 15(4):6609-6624.

Chicago/Turabian Style

Jelonek, Karol; Pietrowska, Monika; Ros, Malgorzata; Zagdanski, Adam; Suchwalko, Agnieszka; Polanska, Joanna; Marczyk, Michal; Rutkowski, Tomasz; Skladowski, Krzysztof; Clench, Malcolm R.; Widlak, Piotr. 2014. "Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients." Int. J. Mol. Sci. 15, no. 4: 6609-6624.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert