Next Article in Journal
GPC1 Regulated by miR-96-5p, Rather than miR-182-5p, in Inhibition of Pancreatic Carcinoma Cell Proliferation
Previous Article in Journal
Neuroprotective Effects of Citicoline in in Vitro Models of Retinal Neurodegeneration
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(4), 6298-6313;

Maternal High Folic Acid Supplement Promotes Glucose Intolerance and Insulin Resistance in Male Mouse Offspring Fed a High-Fat Diet

Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150086, China
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China
Author to whom correspondence should be addressed.
Received: 27 January 2014 / Revised: 3 March 2014 / Accepted: 28 March 2014 / Published: 14 April 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Full-Text   |   PDF [701 KB, uploaded 19 June 2014]


Maternal nutrition may influence metabolic profiles in offspring. We aimed to investigate the effect of maternal folic acid supplement on glucose metabolism in mouse offspring fed a high-fat diet (HFD). Sixty C57BL/6 female mice were randomly assigned into three dietary groups and fed the AIN-93G diet containing 2 (control), 5 (recommended folic acid supplement, RFolS) or 40 (high folic acid supplement, HFolS) mg folic acid/kg of diet. All male offspring were fed HFD for eight weeks. Physiological, biochemical and genetic variables were measured. Before HFD feeding, developmental variables and metabolic profiles were comparable among each offspring group. However, after eight weeks of HFD feeding, the offspring of HFolS dams (Off-HFolS) were more vulnerable to suffer from obesity (p = 0.009), glucose intolerance (p < 0.001) and insulin resistance (p < 0.001), compared with the controls. Off-HFolS had reduced serum adiponectin concentration, accompanied with decreased adiponectin mRNA level but increased global DNA methylation level in white adipose tissue. In conclusion, our results suggest maternal HFolS exacerbates the detrimental effect of HFD on glucose intolerance and insulin resistance in male offspring, implying that HFolS during pregnancy should be adopted cautiously in the general population of pregnant women to avoid potential deleterious effect on the metabolic diseases in their offspring. View Full-Text
Keywords: folate; epigenetics; metabolic syndrome; gestational nutrition supplement folate; epigenetics; metabolic syndrome; gestational nutrition supplement
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Huang, Y.; He, Y.; Sun, X.; He, Y.; Li, Y.; Sun, C. Maternal High Folic Acid Supplement Promotes Glucose Intolerance and Insulin Resistance in Male Mouse Offspring Fed a High-Fat Diet. Int. J. Mol. Sci. 2014, 15, 6298-6313.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top