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Int. J. Mol. Sci. 2014, 15(3), 5140-5162; doi:10.3390/ijms15035140
Article

Structure and Antitumor and Immunomodulatory Activities of a Water-Soluble Polysaccharide from Dimocarpus longan Pulp

1,†
, 1,2,†
, 3
, 4
, 1
, 1
, 1
, 1
 and 1,*
1 School of Pharmaceutical Sciences, Guangxi Medical University, No. 22 Shuangyong Road, Nanning 530021, Guangxi, China 2 Wu Jieping Medical Foundation Cell and Molecular Clinical Research Center, Beihai People's Hospital, No. 83 Heping Road, Beihai 536000, Guangxi, China 3 Department of Pharmacy, Heilongjiang Nursing College, No. 209 Xuefu Road, Harbin 150036, Heilongjiang, China 4 Department of Acupuncture and Moxibustion, the People's Hospital of Guangxi Zhuang Autonomous Region, No. 6 Taoyuan Road, Nanning 530021, Guangxi, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 8 December 2013 / Revised: 30 January 2014 / Accepted: 10 February 2014 / Published: 24 March 2014
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography. The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis. The results indicated that the molecular weight of the sample was 1.1 × 105 Da. Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21. Structural analysis indicated that LP1 consisted of a backbone of →4)-α-d-Glcp-(1→4)-α-d-GalpA-(1→4)-α-d-Glcp-(1→4)-β-d-Glcp-(1→ units with poly saccharide side chains composed of →2)-β-d-Fruf-(1→2)-l-sorbose-(1→ attached to the O-6 position of the α-d-Glcp residues. In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively. In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation. The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
Keywords: structure; antitumor activity; immunomodulatory activity; polysaccharide; Dimocarpus longan pulp structure; antitumor activity; immunomodulatory activity; polysaccharide; Dimocarpus longan pulp
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Meng, F.-Y.; Ning, Y.-L.; Qi, J.; He, Z.; Jie, J.; Lin, J.-J.; Huang, Y.-J.; Li, F.-S.; Li, X.-H. Structure and Antitumor and Immunomodulatory Activities of a Water-Soluble Polysaccharide from Dimocarpus longan Pulp. Int. J. Mol. Sci. 2014, 15, 5140-5162.

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