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Int. J. Mol. Sci. 2014, 15(3), 4031-4048; doi:10.3390/ijms15034031
Article

Role of miR-191/425 Cluster in Tumorigenesis and Diagnosis of Gastric Cancer

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1
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2
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2,*  and 1,*
1 Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China 2 Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
* Authors to whom correspondence should be addressed.
Received: 17 January 2014 / Revised: 18 February 2014 / Accepted: 19 February 2014 / Published: 5 March 2014
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Abstract

Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorigenic role and clinical significance of miR-191/425 overexpression in gastric carcinogenesis is currently undefined. In this study, the expression of miR-191 and miR-425 in GC tissue and serum was assessed, and the relationship between miRNA expression and clinicopathological data was analyzed. We found that miR-191 and miR-425 were both significantly increased in human GC tissues relative to adjacent normal controls. In addition, miR-191 levels correlated with GC tumor stage and metastatic state. Furthermore, the level of serum miR-191 was significantly higher in the GC group than in the control group when using serum miR-16 as an endogenous control. Finally, inhibition of miR-191 or miR-425 in the GC cell lines HGC-27 not only reduced cell proliferation and cell cycle progression but also impaired cell migration and invasion. Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.
Keywords: miR-191; miR-425; serum miRNA; gastric cancer miR-191; miR-425; serum miRNA; gastric cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Peng, W.-Z.; Ma, R.; Wang, F.; Yu, J.; Liu, Z.-B. Role of miR-191/425 Cluster in Tumorigenesis and Diagnosis of Gastric Cancer. Int. J. Mol. Sci. 2014, 15, 4031-4048.

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