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Int. J. Mol. Sci. 2014, 15(3), 3432-3443; doi:10.3390/ijms15033432
Article

Genistein-Inhibited Cancer Stem Cell-Like Properties and Reduced Chemoresistance of Gastric Cancer

1,2
,
3
,
1
,
1
 and
1,*
1 Department of Surgical Oncology, First Affiliated Hospital of Xiamen University & Xiamen Cancer Center, Xiamen 361003, Fujian, China 2 Medical College, China Three Gorges University, Yichang 443002, Hubei, China 3 Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China
* Author to whom correspondence should be addressed.
Received: 4 December 2013 / Revised: 12 February 2014 / Accepted: 13 February 2014 / Published: 25 February 2014
(This article belongs to the Section Molecular Pathology)
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Abstract

Genistein, the predominant isoflavone found in soy products, has exerted its anticarcinogenic effect in many different tumor types in vitro and in vivo. Accumulating evidence in recent years has strongly indicated the existence of cancer stem cells in gastric cancer. Here, we showed that low doses of genistein (15 µM), extracted from Millettia nitida Benth var hirsutissima Z Wei, inhibit tumor cell self-renewal in two types of gastric cancer cells by colony formation assay and tumor sphere formation assay. Treatment of gastric cancer cells with genistein reduced its chemoresistance to 5-Fu (fluorouracil) and ciplatin. Further results indicated that the reduced chemoresistance may be associated with the inhibition of ABCG2 expression and ERK 1/2 activity. Furthermore, genistein reduced tumor mass in the xenograft model. Together, genistein inhibited gastric cancer stem cell-like properties and reduced its chemoresistance. Our results provide a further rationale and experimental basis for using the genistein to improve treatment of patients with gastric cancer.
Keywords: genistein; gastric cancer stem cell; chemoresistance genistein; gastric cancer stem cell; chemoresistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Huang, W.; Wan, C.; Luo, Q.; Huang, Z.; Luo, Q. Genistein-Inhibited Cancer Stem Cell-Like Properties and Reduced Chemoresistance of Gastric Cancer. Int. J. Mol. Sci. 2014, 15, 3432-3443.

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