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Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea
CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal
IPATIMUP—Institute of Molecular Pathology and Immunology of the University of Porto, 4200-465 Porto, Portugal
Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350 Copenhagen, Denmark
Department of Biology, Faculty of Sciences, Porto University, Rua do Campo Alegre, 4069-007 Porto, Portugal
* Author to whom correspondence should be addressed.
Received: 18 November 2013; in revised form: 31 December 2013 / Accepted: 20 January 2014 / Published: 27 January 2014
Abstract: Suspension-feeding bivalves are considered efficient toxin vectors with a relative insensitivity to toxicants compared to other aquatic organisms. This fact highlights the potential role of detoxiﬁcation enzymes, such as glutathione transferases (GSTs), in this bivalve resistance. Nevertheless, the GST system has not been extensively described in these organisms. In the present study, cytosolic GSTs isoforms (cGST) were surveyed in three bivalves with different habitats and life strategies: Corbicula fluminea, Anodonta cygnea and Mytilus galloprovincialis. GSTs were purified by glutathione-agarose afﬁnity chromatography, and the collection of expressed cGST classes of each bivalve were identified using a proteomic approach. All the purified extracts were also characterized kinetically. Results reveal variations in cGST subunits collection (diversity and properties) between the three tested bivalves. Using proteomics, four pi-class and two sigma-class GST subunits were identified in M. galloprovincialis. C. fluminea also yielded four pi-class and one sigma-class GST subunits. For A. cygnea, two mu-class and one pi-class GST subunits were identified, these being the first record of GSTs from these freshwater mussels. The affinity purified extracts also show differences regarding enzymatic behavior among species. The variations found in cGST collection and kinetics might justify diverse selective advantages for each bivalve organism.
Keywords: glutathione transferases; Corbicula fluminea; Anodonta cygnea; Mytilus galloprovincialis; detoxification; proteomics
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Martins, J.C.; Campos, A.; Osório, H.; da Fonseca, R.; Vasconcelos, V. Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea. Int. J. Mol. Sci. 2014, 15, 1887-1900.
Martins JC, Campos A, Osório H, da Fonseca R, Vasconcelos V. Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea. International Journal of Molecular Sciences. 2014; 15(2):1887-1900.
Martins, José C.; Campos, Alexandre; Osório, Hugo; da Fonseca, Rute; Vasconcelos, Vítor. 2014. "Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea." Int. J. Mol. Sci. 15, no. 2: 1887-1900.