Int. J. Mol. Sci. 2014, 15(2), 1887-1900; doi:10.3390/ijms15021887
Article

Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea

1,* email, 1email, 2,3email, 1,4email and 1,5email
Received: 18 November 2013; in revised form: 31 December 2013 / Accepted: 20 January 2014 / Published: 27 January 2014
(This article belongs to the collection Advances in Proteomic Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Suspension-feeding bivalves are considered efficient toxin vectors with a relative insensitivity to toxicants compared to other aquatic organisms. This fact highlights the potential role of detoxification enzymes, such as glutathione transferases (GSTs), in this bivalve resistance. Nevertheless, the GST system has not been extensively described in these organisms. In the present study, cytosolic GSTs isoforms (cGST) were surveyed in three bivalves with different habitats and life strategies: Corbicula fluminea, Anodonta cygnea and Mytilus galloprovincialis. GSTs were purified by glutathione-agarose affinity chromatography, and the collection of expressed cGST classes of each bivalve were identified using a proteomic approach. All the purified extracts were also characterized kinetically. Results reveal variations in cGST subunits collection (diversity and properties) between the three tested bivalves. Using proteomics, four pi-class and two sigma-class GST subunits were identified in M. galloprovincialis. C. fluminea also yielded four pi-class and one sigma-class GST subunits. For A. cygnea, two mu-class and one pi-class GST subunits were identified, these being the first record of GSTs from these freshwater mussels. The affinity purified extracts also show differences regarding enzymatic behavior among species. The variations found in cGST collection and kinetics might justify diverse selective advantages for each bivalve organism.
Keywords: glutathione transferases; Corbicula fluminea; Anodonta cygnea; Mytilus galloprovincialis; detoxification; proteomics
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MDPI and ACS Style

Martins, J.C.; Campos, A.; Osório, H.; da Fonseca, R.; Vasconcelos, V. Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea. Int. J. Mol. Sci. 2014, 15, 1887-1900.

AMA Style

Martins JC, Campos A, Osório H, da Fonseca R, Vasconcelos V. Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea. International Journal of Molecular Sciences. 2014; 15(2):1887-1900.

Chicago/Turabian Style

Martins, José C.; Campos, Alexandre; Osório, Hugo; da Fonseca, Rute; Vasconcelos, Vítor. 2014. "Proteomic Profiling of Cytosolic Glutathione Transferases from Three Bivalve Species: Corbicula fluminea, Mytilus galloprovincialis and Anodonta cygnea." Int. J. Mol. Sci. 15, no. 2: 1887-1900.


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