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Int. J. Mol. Sci. 2014, 15(12), 23269-23282; doi:10.3390/ijms151223269

Identification of Biomarkers in Cerebrospinal Fluid and Serum of Multiple Sclerosis Patients by Immunoproteomics Approach

1
Istituto di Biomedicina e Immunologia Molecolare "A. Monroy", Consiglio Nazionale delle Ricerche (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy
2
Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sez. Biologia e Genetica, Università di Palermo, via Divisi 83, 90139 Palermo, Italy
3
Dipartimento di Biomedicina Sperimentale e Neuroscienze, Università di Palermo, via del Vespro 129, 90127 Palermo, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 3 November 2014 / Revised: 5 December 2014 / Accepted: 8 December 2014 / Published: 15 December 2014
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the central nervous system. At present, the molecular mechanisms causing the initiation, development and progression of MS are poorly understood, and no reliable proteinaceous disease markers are available. In this study, we used an immunoproteomics approach to identify autoreactive antibodies in the cerebrospinal fluid of MS patients to use as candidate markers with potential diagnostic value. We identified an autoreactive anti-transferrin antibody that may have a potential link with the development and progression of MS. We found this antibody at high levels also in the serum of MS patients and created an immunoenzymatic assay to detect it. Because of the complexity and heterogeneity of multiple sclerosis, it is difficult to find a single marker for all of the processes involved in the origin and progression of the disease, so the development of a panel of biomarkers is desirable, and anti-transferrin antibody could be one of these. View Full-Text
Keywords: multiple sclerosis; cerebrospinal fluid; immunoproteome; anti-transferrin autoantibodies; serum biomarker multiple sclerosis; cerebrospinal fluid; immunoproteome; anti-transferrin autoantibodies; serum biomarker
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MDPI and ACS Style

Colomba, P.; Fontana, S.; Salemi, G.; Barranca, M.; Lo Sicco, C.; Mazzola, M.A.; Ragonese, P.; Savettieri, G.; De Leo, G.; Alessandro, R.; Duro, G. Identification of Biomarkers in Cerebrospinal Fluid and Serum of Multiple Sclerosis Patients by Immunoproteomics Approach. Int. J. Mol. Sci. 2014, 15, 23269-23282.

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