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Int. J. Mol. Sci. 2014, 15(12), 23179-23195; doi:10.3390/ijms151223179

Crosstalk between Edc4 and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in mRNA Decapping

1
Institute for Clinical Chemistry/UMG-Laboratories, University Medical Center, Robert-Koch-Str. 40, Goettingen 37075, Germany
2
Department of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan
*
Author to whom correspondence should be addressed.
Received: 22 October 2014 / Revised: 28 November 2014 / Accepted: 8 December 2014 / Published: 12 December 2014
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

The mammalian target of rapamycin complex 1 (mTORC1) is involved in the cellular transcription and translation processes. The undertaken study characterized the enhancer of mRNA decapping protein 4 (Edc4) as mTORC1 interacting protein. Human T lymphoblast (CCRF-CEM) cells were used for mTORC1 purification. Co-immunoprecipitation coupled with immunoblotting analysis was used to confirm the interaction of Edc4 in mTORC1 specific purifications. Further assays were incorporated to conclude the role of mTORC1 in mRNA decapping via Edc4. Edc4 was identified as a new interacting protein with mTORC1 in both the endogenous and myc-tag raptor component mTORC1 specific purifications. Quantitative co-localization using confocal microscopy demonstrated that raptor component of mTORC1 coexists with Edc4 in processing (P) bodies, a site for mRNA degradation. Incubation of cells with rapamycin, a known inhibitor of mTOR kinase activity, increased the total Edc4 protein expression but at the same time decreased the Edc4 interaction with mTORC1. Moreover, rapamycin treatment resulted in a significant decrease in total serine phosphorylated Edc4 protein signal and the total 5'-capped mRNA. These findings provide the first evidence for the pivotal role of mTORC1 in Edc4 regulation. Further in-depth studies are required to get a complete understanding of molecular crosstalk between mTORC1 signaling and mRNA decapping pathway. View Full-Text
Keywords: mammalian target of rapamycin complex 1 (mTORC1) purification; mTORC1 interacting proteins; enhancer of mRNA decapping protein 4 (Edc4); mRNA decapping mammalian target of rapamycin complex 1 (mTORC1) purification; mTORC1 interacting proteins; enhancer of mRNA decapping protein 4 (Edc4); mRNA decapping
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rahman, H.; Qasim, M.; Oellerich, M.; Asif, A.R. Crosstalk between Edc4 and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in mRNA Decapping. Int. J. Mol. Sci. 2014, 15, 23179-23195.

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Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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