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Int. J. Mol. Sci. 2014, 15(12), 22933-22959; doi:10.3390/ijms151222933

Molecular Genetic Variability of Commercial and Wild Accessions of Passion Fruit (Passiflora spp.) Targeting ex Situ Conservation and Breeding

1
Laboratory of Applied Molecular Genetics, Department of Exact and Natural Sciences, State University of Southwest Bahia, Itapetinga 45700-000, Brazil
2
Molecular Biology and Genetic Engineering Center, University of Campinas, CP 6010 Campinas, Campinas 13083-875, Brazil
3
Brazilian Agricultural Research Corporation, Cassava & Fruits, Cruz das Almas 44380-000, Brazil
4
Biotechnology and Genetic Center, Biological Sciences Department, State University of Santa Cruz, Ilhéus 45662-900, Brazil
5
Plant Biology Department, Biology Institute, University of Campinas, Campinas 13083-875, Brazil
*
Author to whom correspondence should be addressed.
Received: 21 August 2014 / Revised: 17 October 2014 / Accepted: 24 October 2014 / Published: 10 December 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Passiflora species are distributed throughout Latin America, and Brazil and Colombia serve as the centers of diversity for this genus. We performed cross-species amplification to evaluate 109 microsatellite loci in 14 Passiflora species and estimated the diversity and genetic structure of Passiflora cincinnata, Passiflora setaceae and Passiflora edulis. A total of 127 accessions, including 85 accessions of P. edulis, a commercial species, and 42 accessions of 13 wild species, were examined. The cross-species amplification was effective for obtaining microsatellite loci (average cross-amplification of 70%). The average number of alleles per locus (five) was relatively low, and the average diversity ranged from 0.52 in P. cincinnata to 0.32 in P. setacea. The Bayesian analyses indicated that the P. cincinnata and P. setacea accessions were distributed into two groups, and the P. edulis accessions were distributed into five groups. Private alleles were identified, and suggestions for core collections are presented. Further collections are necessary, and the information generated may be useful for breeding and conservation. View Full-Text
Keywords: germplasm; genetic diversity; Passifloraceae; molecular markers; single sequence repeats germplasm; genetic diversity; Passifloraceae; molecular markers; single sequence repeats
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cerqueira-Silva, C.B.M.; Santos, E.S.L.; Jesus, O.N.; Vieira, J.G.P.; Mori, G.M.; Corrêa, R.X.; Souza, A.P. Molecular Genetic Variability of Commercial and Wild Accessions of Passion Fruit (Passiflora spp.) Targeting ex Situ Conservation and Breeding. Int. J. Mol. Sci. 2014, 15, 22933-22959.

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