Next Article in Journal
Multigenerational Study of Chemically Induced Cytotoxicity and Proliferation in Cultures of Human Proximal Tubular Cells
Next Article in Special Issue
MiR-222 Targeted PUMA to Improve Sensitization of UM1 Cells to Cisplatin
Previous Article in Journal
Inhibition of p53 deSUMOylation Exacerbates Puromycin Aminonucleoside-Induced Apoptosis in Podocytes
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(11), 21331-21347; doi:10.3390/ijms151121331

Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines

1,2,3,†
,
2,†
,
2,* , 4
and
1,2,*
1
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
2
Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences, Shenzhen University, Shenzhen 518060, China
3
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China
4
Microorganism Examination Division, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 18 September 2014 / Revised: 7 November 2014 / Accepted: 7 November 2014 / Published: 18 November 2014
(This article belongs to the Special Issue RNA Interference)
View Full-Text   |   Download PDF [2122 KB, uploaded 18 November 2014]   |  

Abstract

Apoptosis, as a programmed cell death process, is essential for the maintenance of tissue function in organisms. Alteration of this process is linked to many diseases. Over-expression of clusterin (Clu) can antagonize apoptosis in various cells. Selenium (Se) is an essential trace element for human health. Its biological function is also associated with cell apoptosis. To explore the function of Clu and the impact of Se in the process of apoptosis, several short-hairpin RNAs (shRNA) were designed for the construction of two sets of recombinant plasmids: one set for plasmid-transfection of mouse neuroblastoma N2a cells (N2a cells); and the other set for lentiviral infection of human neuroblastoma SH-SY5Y cells (SH-SY5Y cells). These shRNAs specifically and efficiently interfered with the intracellular expression of Clu at both the mRNA and protein levels. The Clu-knockdown cells showed apoptosis-related features, including down-regulation of antioxidative capacity and the Bcl-2/Bax ratio and up-regulation of caspase-8 activity. Se-methylselenocysteine (MSC) at an optimum concentration of 1 μM could reverse the alteration in antioxidative capacity, Bcl2/Bax ratio and caspase-8 activity caused by Clu-knockdown, thus inhibiting apoptosis and maintaining cell viability. The results hereby imply the potentiality of Clu and Se in neuroprotection. View Full-Text
Keywords: clusterin (Clu); Se-methylselenocysteine (MSC); cell apoptosis; RNA interference (RNAi); gene knockdown clusterin (Clu); Se-methylselenocysteine (MSC); cell apoptosis; RNA interference (RNAi); gene knockdown
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, C.; Zeng, Z.; Liu, Q.; Zhang, R.; Ni, J. Se-Methylselenocysteine Inhibits Apoptosis Induced by Clusterin Knockdown in Neuroblastoma N2a and SH-SY5Y Cell Lines. Int. J. Mol. Sci. 2014, 15, 21331-21347.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top