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Int. J. Mol. Sci. 2014, 15(11), 21215-21228; doi:10.3390/ijms151121215

Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii

Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, Guangdong, China
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Received: 23 August 2014 / Revised: 10 October 2014 / Accepted: 11 November 2014 / Published: 17 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encoding a 429 amino acid polypeptide and a 3'-UTR of 1388 bp. The deduced protein sequence of Pm-BMP7 contained a signal peptide, a pro-domain and a mature peptide. The mature peptide consisted of 135 amino acids and included a transforming growth factor β family domain with six shared cysteine residues. The protein sequence of Pm-BMP7 showed 66% identity with that from Crassostrea gigas. Two unigenes encoding Pm-BMPRI (Pm-BMP receptor I) and Pm-BMPRII were obtained from the transcriptome database of P. martensii. Tissue expression analysis demonstrated Pm-BMP7 and Pm-BMPRI were highly expressed in the mantle (shell formation related-tissue), while Pm-BMPRII was highly expressed in the foot. After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue). The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group. These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell. View Full-Text
Keywords: Pinctada martensii; bone morphogenetic protein 7 (BMP7); RNA interference; nacre; prismatic layer; shell Pinctada martensii; bone morphogenetic protein 7 (BMP7); RNA interference; nacre; prismatic layer; shell
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yan, F.; Luo, S.; Jiao, Y.; Deng, Y.; Du, X.; Huang, R.; Wang, Q.; Chen, W. Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii. Int. J. Mol. Sci. 2014, 15, 21215-21228.

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