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Int. J. Mol. Sci. 2014, 15(11), 20538-20554; doi:10.3390/ijms151120538

Intracellular Signaling Transduction Pathways Triggered by a Well-Known Anti-GHR Monoclonal Antibody, Mab263, in Vitro and in Vivo

1
College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun 130118, China
2
State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 15 September 2014 / Revised: 21 October 2014 / Accepted: 28 October 2014 / Published: 10 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

A series of studies have reported that monoclonal antibody 263 (Mab263), a monoclonal antibody against the growth hormone receptor (GHR), acts as an agonist in vitro and in vivo. However, the intracellular signaling pathways triggered by Mab263 have not yet been delineated. Therefore, we examined the intracellular signaling pathways induced by Mab263 in vivo and in vitro in the present study. The results show that this antibody activated janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), STAT1 and extracellular signal-regulated kinase 1/2 (ERK1/2), but not STAT5. The phosphorylation kinetics of JAK2, STAT3/1 and ERK1/2 induced by Mab263 were subsequently analyzed in dose-response and time course experiments. Our observations indicate that Mab263 induced different intracellular signaling pathways than GH, which indicates that Mab263 is a signal-specific molecule and that Mab263 may be a valuable biological reagent to study the mechanism(s) of GHR-mediated intracellular signaling pathways. View Full-Text
Keywords: monoclonal antibody 263 (Mab263); growth hormone receptor; signal transduction pathway monoclonal antibody 263 (Mab263); growth hormone receptor; signal transduction pathway
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lan, H.; Li, W.; Jiang, H.; Yang, Y.; Zheng, X. Intracellular Signaling Transduction Pathways Triggered by a Well-Known Anti-GHR Monoclonal Antibody, Mab263, in Vitro and in Vivo. Int. J. Mol. Sci. 2014, 15, 20538-20554.

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