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Int. J. Mol. Sci. 2014, 15(11), 20382-20402; doi:10.3390/ijms151120382

Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds by HPLC–ESI-QTOF-MS

1
Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071 Granada, Spain
2
Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento s/n, Edificio BioRegion, 18016 Granada, Spain
3
Biomedical Research Centre, University Hospital of Sant Joan, IISPV, Rovira i Virgili University, C/Sant Joan s/n, 43201 Reus (Tarragona), Spain
*
Author to whom correspondence should be addressed.
Received: 28 July 2014 / Revised: 24 September 2014 / Accepted: 28 October 2014 / Published: 6 November 2014
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols)
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Abstract

The consumption of polyphenols has frequently been associated with low incidence of degenerative diseases. Most of these natural antioxidants come from fruits, vegetables, spices, grains and herbs. For this reason, there has been increasing interest in identifying plant extract compounds. Polymeric tannins and monomeric flavonoids, such as catechin and epicatechin, in pine bark and green tea extracts could be responsible for the higher antioxidant activities of these extracts. The aim of the present study was to characterize the phenolic compounds in pine bark and green tea concentrated extracts using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC–ESI-QTOF-MS). A total of 37 and 35 compounds from pine bark and green tea extracts, respectively, were identified as belonging to various structural classes, mainly flavan-3-ol and its derivatives (including procyanidins). The antioxidant capacity of both extracts was evaluated by three complementary antioxidant activity methods: Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). Higher antioxidant activity values by each method were obtained. In addition, total polyphenol and flavan-3-ol contents, which were determined by Folin–Ciocalteu and vanillin assays, respectively, exhibited higher amounts of gallic acid and (+)-catechin equivalents. View Full-Text
Keywords: pine bark; green tea; polyphenols; flavan-3-ols; procyanidins; antioxidant activity; HPLC–ESI-QTOF-MS pine bark; green tea; polyphenols; flavan-3-ols; procyanidins; antioxidant activity; HPLC–ESI-QTOF-MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

de la Luz Cádiz-Gurrea, M.; Fernández-Arroyo, S.; Segura-Carretero, A. Pine Bark and Green Tea Concentrated Extracts: Antioxidant Activity and Comprehensive Characterization of Bioactive Compounds by HPLC–ESI-QTOF-MS. Int. J. Mol. Sci. 2014, 15, 20382-20402.

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