Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis
AbstractN,N'-dinitrosopiperazine (DNP) with organ specificity for nasopharyngeal epithelium, is involved in nasopharyngeal carcinoma (NPC) metastasis, though its mechanism is unclear. To reveal the pathogenesis of DNP-induced metastasis, immunoprecipitation was used to identify DNP-mediated phosphoproteins. DNP-mediated NPC cell line (6-10B) motility and invasion was confirmed. Twenty-six phosphoproteins were increased at least 1.5-fold following DNP exposure. Changes in the expression levels of selected phosphoproteins were verified by Western-blotting analysis. DNP treatment altered the phosphorylation of ezrin (threonine 567), vimentin (serine 55), stathmin (serine 25) and STAT3 (serine 727). Furthermore, it was shown that DNP-dependent metastasis is mediated in part through ezrin at threonine 567, as DNP-mediated metastasis was decreased when threonine 567 of ezrin was mutated. Strikingly, NPC metastatic tumors exhibited a higher expression of phosphorylated-ezrin at threonine 567 than the primary tumors. These findings provide novel insight into DNP-induced NPC metastasis and may contribute to a better understanding of the metastatic mechanisms of NPC tumors. View Full-Text
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Tan, G.; Tang, X.; Huang, D.; Li, Y.; Liu, N.; Peng, Z.; Zhang, Z.; Duan, C.; Lu, J.; Yan, G.; Tang, F. Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis. Int. J. Mol. Sci. 2014, 15, 20054-20071.
Tan G, Tang X, Huang D, Li Y, Liu N, Peng Z, Zhang Z, Duan C, Lu J, Yan G, Tang F. Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis. International Journal of Molecular Sciences. 2014; 15(11):20054-20071.Chicago/Turabian Style
Tan, Gongjun; Tang, Xiaowei; Huang, Damao; Li, Yuejin; Liu, Na; Peng, Zhengke; Zhang, Zhenlin; Duan, Chaojun; Lu, Jinping; Yan, Guangrong; Tang, Faqing. 2014. "Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis." Int. J. Mol. Sci. 15, no. 11: 20054-20071.