Next Article in Journal
ZmSOC1, a MADS-Box Transcription Factor from Zea mays, Promotes Flowering in Arabidopsis
Next Article in Special Issue
Contrast Agents for Photoacoustic and Thermoacoustic Imaging: A Review
Previous Article in Journal
Predictive Value of Decoy Receptor 3 in Postoperative Nosocomial Bacterial Meningitis
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(11), 19971-19986; doi:10.3390/ijms151119971

Ultrasensitive Imaging of Ca2+ Dynamics in Pancreatic Acinar Cells of Yellow Cameleon-Nano Transgenic Mice

1
Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Toon City, Ehime 791-0295, Japan
2
Division of Bio-Imaging, Proteo-Science Center, Ehime University, Toon City, Ehime 791-0295, Japan
3
Translational Research Center, Ehime University Hospital, Toon City, Ehime 791-0295, Japan
4
Laboratory for Spatiotemporal Regulations, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan
5
Laboratory of Molecular and Cellular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan
6
The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan
*
Authors to whom correspondence should be addressed.
Received: 9 September 2014 / Revised: 22 October 2014 / Accepted: 28 October 2014 / Published: 3 November 2014
(This article belongs to the Special Issue Laser Application in Life Sciences)
View Full-Text   |   Download PDF [6825 KB, uploaded 3 November 2014]   |  

Abstract

Yellow Cameleons are genetically encoded Ca2+ indicators in which cyan and yellow fluorescent proteins and calmodulin work together as a fluorescence (Förster) resonance energy transfer Ca2+-sensor probe. To achieve ultrasensitive Ca2+ imaging for low resting Ca2+ or small Ca2+ transients in various organs, we generated a transgenic mouse line expressing the highest-sensitive genetically encoded Ca2+ indicator (Yellow Cameleon-Nano 15) in the whole body. We then focused on the mechanism of exocytotic events mediated by intracellular Ca2+ signaling in acinar cells of the mice with an agonist and observed them by two-photon excitation microscopy. In the results, two-photon excitation imaging of Yellow Cameleon-Nano 15 successfully visualized intracellular Ca2+ concentration under stimulation with the agonist at nanomolar levels. This is the first demonstration for application of genetically encoded Ca2+ indicators to pancreatic acinar cells. We also simultaneously observed exocytotic events and an intracellular Ca2+ concentration under in vivo condition. Yellow Cameleon-Nano 15 mice are healthy and no significant deteriorative effect was observed on physiological response regarding the pancreatic acinar cells. The dynamic range of 165% was calculated from Rmax and Rmin values under in vivo condition. The mice will be useful for ultrasensitive Ca2+ imaging in vivo. View Full-Text
Keywords: Yellow Cameleon; Ca2+ indicator; Ca2+ imaging; Förster resonance energy transfer (FRET); genetically encoded Ca2+ indicators (GECI); Yellow Cameleon-Nano (YC-Nano); two-photon excitation fluorescence microscopy; live-cell imaging; exocytotsis; acinar cell Yellow Cameleon; Ca2+ indicator; Ca2+ imaging; Förster resonance energy transfer (FRET); genetically encoded Ca2+ indicators (GECI); Yellow Cameleon-Nano (YC-Nano); two-photon excitation fluorescence microscopy; live-cell imaging; exocytotsis; acinar cell
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Oshima, Y.; Imamura, T.; Shintani, A.; Kajiura-Kobayashi, H.; Hibi, T.; Nagai, T.; Nonaka, S.; Nemoto, T. Ultrasensitive Imaging of Ca2+ Dynamics in Pancreatic Acinar Cells of Yellow Cameleon-Nano Transgenic Mice. Int. J. Mol. Sci. 2014, 15, 19971-19986.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top