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Int. J. Mol. Sci. 2014, 15(11), 19516-19534;

X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood

Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou 450052, China
College of Public Health, Zhengzhou University, Zhengzhou 450001, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 24 August 2014 / Revised: 16 October 2014 / Accepted: 20 October 2014 / Published: 27 October 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Using quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood. View Full-Text
Keywords: X-ray; human peripheral blood; inflammation-related genes; quantitative PCR array X-ray; human peripheral blood; inflammation-related genes; quantitative PCR array

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Wang, P.; Guo, F.; Han, L.; Wang, X.; Li, J.; Guo, Y.; LÜ, Y. X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood. Int. J. Mol. Sci. 2014, 15, 19516-19534.

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