X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood
AbstractUsing quantitative real-time polymerase chain reaction (PCR) array, we explored and compared the expression changes of inflammation-related genes in human peripheral blood irradiated with 0.5, 3, and 10 Gy doses of X-rays 24 h after exposure. Results indicated that the expression of 62 out of 84 genes was significantly altered after X-ray radiation. Among these 62 genes, 35 (such as TNFSF4) are known to be associated with radiation response, but others are novel. At a low radiation dose (0.5 Gy), 9 genes were up-regulated and 19 were down-regulated. With further increased dose to 3 Gy, 8 unique genes were up-regulated and 19 genes were down-regulated. We also identified 48 different genes that were differentially expressed significantly after 10 Gy of irradiation, and among these transcripts, up-regulated genes accounted for only one-third (16 genes) of the total. Of the 62 genes, 31 were significantly altered only at a specific dose, and a total of 10 genes were significantly expressed at all 3 doses. The dose- and time-dependent expression of CCL2 was confirmed by quantitative real-time reverse-transcription PCR. A number of candidate genes reported herein may be useful molecular biomarkers of radiation exposure in human peripheral blood. View Full-Text
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Wang, P.; Guo, F.; Han, L.; Wang, X.; Li, J.; Guo, Y.; LÜ, Y. X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood. Int. J. Mol. Sci. 2014, 15, 19516-19534.
Wang P, Guo F, Han L, Wang X, Li J, Guo Y, LÜ Y. X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood. International Journal of Molecular Sciences. 2014; 15(11):19516-19534.Chicago/Turabian Style
Wang, Ping; Guo, Fei; Han, Lin; Wang, Xi'ai; Li, Jie; Guo, Yan; LÜ, Yumin. 2014. "X-ray-Induced Changes in the Expression of Inflammation-Related Genes in Human Peripheral Blood." Int. J. Mol. Sci. 15, no. 11: 19516-19534.