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Int. J. Mol. Sci. 2014, 15(11), 19394-19405; doi:10.3390/ijms151119394

Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression

1
Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
2
Department of Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China
*
Author to whom correspondence should be addressed.
Received: 4 September 2014 / Revised: 30 September 2014 / Accepted: 2 October 2014 / Published: 24 October 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Catalpol is expected to possess diverse pharmacological actions including anti-cancer, anti-inflammatory and hypoglycemic properties. Matrix metalloproteinase-2 (MMP-2) is closely related to the pathogenesis of ovarian cancer. In addition, microRNA-200 (miR-200) can modulate phenotype, proliferation, infiltration and transfer of various tumors. Here, OVCAR-3 cells were employed to investigate whether the effect of catalpol (25, 50 and 100 μg/mL) promoted apoptosis of ovarian cancer cells and to explore the potential mechanisms. Our results demonstrate that catalpol could remarkably reduce the proliferation and accelerate the apoptosis of OVCAR-3 cells. Interestingly, our findings show that catalpol treatment significantly decreased the MMP-2 protein level and increased the miR-200 expression level in OVCAR-3 cells. Further, microRNA-200 was shown to regulate the protein expression of MMP-2 in OVCAR-3 cells. It is concluded that catalpol suppressed cellular proliferation and accelerated apoptosis in OVCAR-3 ovarian cancer cells via promoting microRNA-200 expression levels and restraining MMP-2 signaling. View Full-Text
Keywords: catalpol; ovarian cancer; OVCAR-3 cell; MicroRNA-200; matrix metalloproteinase-2 catalpol; ovarian cancer; OVCAR-3 cell; MicroRNA-200; matrix metalloproteinase-2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gao, N.; Tian, J.-X.; Shang, Y.-H.; Zhao, D.-Y.; Wu, T. Catalpol Suppresses Proliferation and Facilitates Apoptosis of OVCAR-3 Ovarian Cancer Cells through Upregulating MicroRNA-200 and Downregulating MMP-2 Expression. Int. J. Mol. Sci. 2014, 15, 19394-19405.

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