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Int. J. Mol. Sci. 2014, 15(10), 19147-19161; doi:10.3390/ijms151019147

Identification and Molecular Characterization of a Chitin-Binding Protein from the Beet Webworm, Loxostege sticticalis L.

1
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China
*
Authors to whom correspondence should be addressed.
Received: 29 August 2014 / Revised: 26 September 2014 / Accepted: 13 October 2014 / Published: 22 October 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

As the first crucial barrier in the midgut of insects, the peritrophic membrane (PM) plays an important role in preventing external invasion. PM proteins, as the major components of the PM, determine the structure and function of this membrane. A new PM protein, named LstiCBP, from the PM of Loxostege sticticalis larvae was identified using cDNA library screening. The full cDNA of LstiCBP is 2606 bp in length and contains a 2403 bp ORF that encodes an 808-amino acid preprotein with a 15-amino acid as signal peptide. The deduced protein sequence of the cDNA contains 8 cysteine-rich chitin-binding domains (CBDs). Recombinant LstiCBP was successfully expressed in BL21 cells using recombinant plasmid DNA and showed high chitin-binding activity. LstiCBP expression was detected in the midgut at both the transcriptional and translational levels; however, the biochemical and physiological functions of LstiCBP in L. sticticalis require further investigation. View Full-Text
Keywords: peritrophic membrane protein; Loxostege sticticalis; chitin-binding protein; LstiCBP peritrophic membrane protein; Loxostege sticticalis; chitin-binding protein; LstiCBP
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yin, J.; Yang, S.; Li, K.; Guo, W.; Cao, Y. Identification and Molecular Characterization of a Chitin-Binding Protein from the Beet Webworm, Loxostege sticticalis L.. Int. J. Mol. Sci. 2014, 15, 19147-19161.

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