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Int. J. Mol. Sci. 2014, 15(1), 758-773; doi:10.3390/ijms15010758

Increased Exosomal MicroRNA-21 and MicroRNA-146a Levels in the Cervicovaginal Lavage Specimens of Patients with Cervical Cancer

Department of Obstetrics and Gynecology, Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 8 November 2013 / Revised: 12 December 2013 / Accepted: 16 December 2013 / Published: 8 January 2014
(This article belongs to the Special Issue Advances in Cancer Diagnosis)
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Abstract

Well-run screening programs for cervical cancer in the population at risk have been shown to result in a sharp decrease in the incidence and mortality of cervical cancer in a number of large populations. Expression patterns of a recently identified biomarker family, microRNA, appear to be characteristic of tumor type and developmental origin. Several tumors have been reported to actively release exosomes carrying microRNAs. The present study has determined the association of microRNAs with cervical cancer-derived exosomes. The cervical cancer-derived exosomes were enriched in the cervicovaginal lavages specimens and the abundance of exosomes and exosomal microRNAs was detected by electron microscopy, western blot analysis, RT-qPCR and microRNA target reporter vector. The microRNA-21 and microRNA-146a, which were up-regulated in cervical cancer patients, were associated with the high levels of cervical cancer-derived exosomes. In conclusion, we demonstrated the abundance of exosomes in the cervicovaginal lavage specimens of women with cervical cancer. Furthermore, our results indicated that abnormally high levels of microRNA-21 and microRNA-146a existed in the cervical cancer-derived exosomes and the two microRNAs were functional in 293T cells.
Keywords: cervical cancer; exosomes; microRNA-21; microRNA-146a cervical cancer; exosomes; microRNA-21; microRNA-146a
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Liu, J.; Sun, H.; Wang, X.; Yu, Q.; Li, S.; Yu, X.; Gong, W. Increased Exosomal MicroRNA-21 and MicroRNA-146a Levels in the Cervicovaginal Lavage Specimens of Patients with Cervical Cancer. Int. J. Mol. Sci. 2014, 15, 758-773.

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