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Int. J. Mol. Sci. 2014, 15(1), 670-683; doi:10.3390/ijms15010670
Article

Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose

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Received: 1 October 2013; in revised form: 25 December 2013 / Accepted: 27 December 2013 / Published: 7 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Primers based on the cDNA sequence of the goose growth hormone (GH) gene in GenBank were designed to amplify exon 2 of the GH gene in Huoyan goose. A total of 552 individuals were brooded in one batch and raised in Liaoning and Jiangsu Provinces, China. Single nucleotide polymorphisms (SNPs) of exon 2 in the GH gene were detected by the polymerase chain reaction (single strand conformation polymorphism method). Homozygotes were subsequently cloned, sequenced and analyzed. Two SNP mutations were detected, and 10 genotypes (referred to as AA, BB, CC, DD, AB, AC, AD, BC, BD and CD) were obtained. Allele D was predominant, and the frequencies of the 10 genotypes fit the Hardy-Weinberg equilibrium in the male, female and whole populations according to the chi-square test. Based on SNP types, the 10 genotypes were combined into three main genotypes. Multiple comparisons were carried out between different genotypes and production traits when the geese were 10 weeks old. Some indices of production performance were significantly (p < 0.05) associated with the genotype. Particularly, geese with genotype AB or BB were highly productive. Thus, these genotypes may serve as selection markers for production traits in Huoyan geese.
Keywords: multiple comparisons; growth hormone gene; Huoyan goose; production performance; single strand conformation polymorphism multiple comparisons; growth hormone gene; Huoyan goose; production performance; single strand conformation polymorphism
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, Y.; Zhu, Z.; Xu, Q.; Chen, G. Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose. Int. J. Mol. Sci. 2014, 15, 670-683.

AMA Style

Zhang Y, Zhu Z, Xu Q, Chen G. Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose. International Journal of Molecular Sciences. 2014; 15(1):670-683.

Chicago/Turabian Style

Zhang, Yang; Zhu, Zhen; Xu, Qi; Chen, Guohong. 2014. "Association of Polymorphisms of Exon 2 of the Growth Hormone Gene with Production Performance in Huoyan Goose." Int. J. Mol. Sci. 15, no. 1: 670-683.



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