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Int. J. Mol. Sci. 2014, 15(1), 629-653; doi:10.3390/ijms15010629
Article

Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana

1
, 2
, 1
, 2
 and 1,3,*
Received: 29 November 2013; in revised form: 20 December 2013 / Accepted: 24 December 2013 / Published: 6 January 2014
(This article belongs to the collection G Protein-Coupled Receptor Signaling and Regulation)
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Abstract: We have isolated a cDNA coding for a putative invertebrate-type dopamine receptor (Peadop2) from P. americana brain by using a PCR-based strategy. The mRNA is present in samples from brain and salivary glands. We analyzed the distribution of the PeaDOP2 receptor protein with specific affinity-purified polyclonal antibodies. On Western blots, PeaDOP2 was detected in protein samples from brain, subesophageal ganglion, thoracic ganglia, and salivary glands. In immunocytochemical experiments, we detected PeaDOP2 in neurons with their somata being located at the anterior edge of the medulla bilaterally innervating the optic lobes and projecting to the ventro-lateral protocerebrum. In order to determine the functional and pharmacological properties of the cloned receptor, we generated a cell line constitutively expressing PeaDOP2. Activation of PeaDOP2-expressing cells with dopamine induced an increase in intracellular cAMP. In contrast, a C-terminally truncated splice variant of this receptor did not exhibit any functional property by itself. The molecular and pharmacological characterization of the first dopamine receptor from P. americana provides the basis for forthcoming studies focusing on the significance of the dopaminergic system in cockroach behavior and physiology.
Keywords: biogenic amine; cellular signaling; dopamine; G-protein-coupled receptor; insect; salivary gland biogenic amine; cellular signaling; dopamine; G-protein-coupled receptor; insect; salivary gland
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Troppmann, B.; Balfanz, S.; Krach, C.; Baumann, A.; Blenau, W. Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana. Int. J. Mol. Sci. 2014, 15, 629-653.

AMA Style

Troppmann B, Balfanz S, Krach C, Baumann A, Blenau W. Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana. International Journal of Molecular Sciences. 2014; 15(1):629-653.

Chicago/Turabian Style

Troppmann, Britta; Balfanz, Sabine; Krach, Christian; Baumann, Arnd; Blenau, Wolfgang. 2014. "Characterization of an Invertebrate-Type Dopamine Receptor of the American Cockroach, Periplaneta americana." Int. J. Mol. Sci. 15, no. 1: 629-653.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert