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Int. J. Mol. Sci. 2014, 15(1), 15-28; doi:10.3390/ijms15010015
Article

Heat-killed VSL#3 Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Experimental Colitis in Rats

1
,
2
,
2
,
1
,
2
 and
2,*
1 Department of Cadre Ward II, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China 2 Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China
* Author to whom correspondence should be addressed.
Received: 10 October 2013 / Revised: 9 December 2013 / Accepted: 10 December 2013 / Published: 19 December 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

To determine the effects of heat-killed VSL#3 (B. breve, B. longum and B. infantis; L. plantarum, L. bulgaricus, L. casei and L. acidophilus; S. salivarius subsp. thermophilus) therapy in the dextran sulfate sodium (DSS)-induced acute experimental colitis in rats. Acute experimental colitis was induced in rats by 5% DSS and freely drink for seven days. Beginning on Day 8, rats underwent gavage once daily for seven days with heat-killed probiotic VSL#3 (0.6 g/kg/day), colonic damage was evaluated histologically and biochemically seven days after gavage. Expression of inflammatory related mediators (STAT3, P-STAT3) and cytokines (IL-6, IL-23, TGFβ) in colonic tissue were detected. The results revealed that heat-killed and live VSL#3 have identical anti-inflammatory properties by the assessed DAI (disease activity index), colon length, histological tissue and MPO activity. Heat-killed and live VSL#3 results in reduced IL-6, IL-23, TGFβ, STAT3 and P-STAT3 expression in colonic tissue. Heat-killed and live VSL#3 have showed the similar anti-inflammatory activity by inhibiting IL-6/STAT3 pathway in the DSS-induced acute experimental colitis in rats.
Keywords: probiotic; ulcerative colitis; inflammatory bowel disease; experimental colitis; IL-6/STAT3 probiotic; ulcerative colitis; inflammatory bowel disease; experimental colitis; IL-6/STAT3
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sang, L.-X.; Chang, B.; Dai, C.; Gao, N.; Liu, W.-X.; Jiang, M. Heat-killed VSL#3 Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Experimental Colitis in Rats. Int. J. Mol. Sci. 2014, 15, 15-28.

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