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Int. J. Mol. Sci. 2013, 14(9), 17608-17617; doi:10.3390/ijms140917608
Brief Report

Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K

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Received: 9 July 2013; in revised form: 19 July 2013 / Accepted: 22 July 2013 / Published: 28 August 2013
(This article belongs to the Section Material Sciences and Nanotechnology)
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Abstract: Seeding is a versatile method for optimizing crystal growth. Coupling this technique with capillary counter diffusion crystallization enhances the size and diffraction quality of the crystals. In this article, crystals for organic solvent-tolerant recombinant elastase strain K were successfully produced through microseeding with capillary counter-diffusion crystallization. This technique improved the nucleation success rate with a low protein concentration (3.00 mg/mL). The crystal was grown in 1 M ammonium phosphate monobasic and 0.1 M sodium citrate tribasic dihydrate pH 5.6. The optimized crystal size was 1 × 0.1 × 0.05 mm3. Elastase strain K successfully diffracted up to 1.39 Å at SPring-8, Japan, using synchrotron radiation for preliminary data diffraction analysis. The space group was determined to be monoclinic space group P1211 with unit cell parameters of a = 38.99 Ǻ, b = 90.173 Å and c = 40.60 Å.
Keywords: elastase strain K; organic solvent tolerant; seeding technique; capillary counter diffusion elastase strain K; organic solvent tolerant; seeding technique; capillary counter diffusion
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ali, M.S.M.; Said, Z.S.A.M.; Rahman, R.N.Z.R.A.; Chor, A.L.T.; Basri, M.; Salleh, A.B. Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K. Int. J. Mol. Sci. 2013, 14, 17608-17617.

AMA Style

Ali MSM, Said ZSAM, Rahman RNZRA, Chor ALT, Basri M, Salleh AB. Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K. International Journal of Molecular Sciences. 2013; 14(9):17608-17617.

Chicago/Turabian Style

Ali, Mohd S.M.; Said, Zatty S.A.M.; Rahman, Raja N.Z.R.A.; Chor, Adam L.T.; Basri, Mahiran; Salleh, Abu B. 2013. "Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K." Int. J. Mol. Sci. 14, no. 9: 17608-17617.


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