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Int. J. Mol. Sci. 2013, 14(8), 16672-16684; doi:10.3390/ijms140816672
Article

Molecular and Functional Analyses of the Fast Skeletal Myosin Light Chain2 Gene of the Korean Oily Bitterling, Acheilognathus koreensis

1,* , 1
, 1
, 1
, 1
, 1
, 2
 and 3,*
1 Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea 2 Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon 305-719, Korea 3 Department of Molecular Biology, Pusan National University, Busan 609-735, Korea
* Authors to whom correspondence should be addressed.
Received: 13 June 2013 / Revised: 26 July 2013 / Accepted: 1 August 2013 / Published: 13 August 2013
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

We identified and characterized the primary structure of the Korean oily bitterling Acheilognathus koreensis fast skeletal myosin light chain 2 (Akmlc2f), gene. Encoded by seven exons spanning 3955 bp, the deduced 168-amino acid AkMLC2f polypeptide contained an EF-hand calcium-binding motif and showed strong homology (80%–98%) with the MLC2 proteins of Ictalurus punctatus and other species, including mammals. Akmlc2f mRNA was highly enriched in skeletal muscles, and was detectable in other tissues. The upstream regions of Akmlc2f included a TATA box, one copy of a putative MEF-2 binding site and several putative C/EBPβ binding sites. The functional activity of the promoter region of Akmlc2f was examined using luciferase and red fluorescent protein reporters. The Akmlc2f promoter-driven reporter expressions were detected and increased by the C/EBPβ transcription factor in HEK293T cells. The activity of the promoter of Akmlc2f was also confirmed in the developing zebrafish embryo. Although the detailed mechanism underlying the expression of Akmlc2f remains unknown, these results suggest the muscle-specific expression of Akmlc2f transcript and the functional activation of Akmlc2f promoter by C/EBPβ.
Keywords: Acheilognathus koreenis; C/EBPβ; expression; Korean oily bitterling; myosin light chain 2; promoter assay Acheilognathus koreenis; C/EBPβ; expression; Korean oily bitterling; myosin light chain 2; promoter assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kong, H.J.; Lee, Y.-J.; Kim, W.-J.; Kim, H.S.; Kim, B.-S.; An, C.M.; Yeo, S.-Y.; Cho, H.K. Molecular and Functional Analyses of the Fast Skeletal Myosin Light Chain2 Gene of the Korean Oily Bitterling, Acheilognathus koreensis. Int. J. Mol. Sci. 2013, 14, 16672-16684.

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