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Int. J. Mol. Sci. 2013, 14(8), 16040-16057; doi:10.3390/ijms140816040
Article

Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells

1
, 2
, 2
 and 1,2,3,*
Received: 17 May 2013; in revised form: 12 July 2013 / Accepted: 18 July 2013 / Published: 31 July 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Here, we report a natural chemical Matrine, which exhibits anti-melanoma potential with its PTEN activation mechanism. Matrine effectively inhibited proliferation of several carcinoma cell lines, including melanoma V600EBRAF harboring M21 cells. Flow cytometry analysis showed Matrine induced G0/G1 cell cycle arrest in M21 cells dose-dependently. Apoptosis in M21 cells induced by Matrine was identified by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis and Annexin-V/FITC staining. Molecular mechanistic study suggested that Matrine upregulated both mRNA level and protein expression level of phosphatase and tensin homolog deleted on chromosome ten (PTEN), leading to inhibition of the PI3K/Akt pathway. Downregulation of phosphor-Aktser473 by Matrine activated p21 and Bax, which contributed to G0/G1 cell cycle and apoptosis. Besides, Matrine enhanced the PI3K/Akt inhibition effects to inhibit the cell proliferation with PI3K inhibitor, LY2940002. In summary, our findings suggest Matrine is a promising antitumor drug candidate with its possible PTEN activation mechanisms for treating cancer diseases, such as melanomas.
Keywords: Matrine; cell cycle arrest; apoptosis; V600EBRAF melanoma; PTEN Matrine; cell cycle arrest; apoptosis; V600EBRAF melanoma; PTEN
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Jin, H.; Sun, Y.; Wang, S.; Cheng, X. Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells. Int. J. Mol. Sci. 2013, 14, 16040-16057.

AMA Style

Jin H, Sun Y, Wang S, Cheng X. Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells. International Journal of Molecular Sciences. 2013; 14(8):16040-16057.

Chicago/Turabian Style

Jin, Hui; Sun, Yu; Wang, Shuiying; Cheng, Xiaodong. 2013. "Matrine Activates PTEN to Induce Growth Inhibition and Apoptosis in V600EBRAF Harboring Melanoma Cells." Int. J. Mol. Sci. 14, no. 8: 16040-16057.


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