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Int. J. Mol. Sci. 2013, 14(7), 14872-14891; doi:10.3390/ijms140714872
Article

Ectopic Expression of BraYAB1-702, a Member of YABBY Gene Family in Chinese Cabbage, Causes Leaf Curling, Inhibition of Development of Shoot Apical Meristem and Flowering Stage Delaying in Arabidopsis thaliana

1,2,3,†
, 1,2,3,†
, 1,2,3
 and 1,2,3,*
Received: 3 April 2013; in revised form: 29 May 2013 / Accepted: 4 July 2013 / Published: 16 July 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: YABBY gene family plays an important role in the polarity development of lateral organs. We isolated the BraYAB1-702 gene, a member of the YABBY gene family, from young leaves of Chinese cabbage line 06J45. The full-length gene has a 937 bp CDNA sequence and contains an open reading frame (ORF) of 702 bp. The subcellular localization analysis showed that the expression product of the gene was localized in the nucleus. Ectopic expression of BraYAB1-702 in Arabidopsis thaliana caused leaf curling from the adaxial epidermises to abaxial epidermises; the partial abaxialization of the adaxial epidermises of leaves; leaf trichomes and stomata numbers being significantly increased; the plants being severely stunted; the flowering stage being remarkably delayed and inhibiting the development of shoot apical meristem (SAM) with the down-regulation of the expression of SHOOT MERISTEMLESS (STM), Brevipedicellus (BP) and KNAT2 which were related to the development of shoot apical meristem. These results from the present research help to reveal the molecular mechanism of BraYAB1-702 gene in the establishment of adaxial–abaxial polarity of the lateral organs in Chinese cabbage.
Keywords: Chinese cabbage (Brassica campestris L. ssp. pekinensis (Lour.) Olsson); YABBY gene family; quantitative analysis; subcellular localization; ectopic expression Chinese cabbage (Brassica campestris L. ssp. pekinensis (Lour.) Olsson); YABBY gene family; quantitative analysis; subcellular localization; ectopic expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, X.-L.; Yang, Z.-P.; Zhang, J.; Zhang, L.-G. Ectopic Expression of BraYAB1-702, a Member of YABBY Gene Family in Chinese Cabbage, Causes Leaf Curling, Inhibition of Development of Shoot Apical Meristem and Flowering Stage Delaying in Arabidopsis thaliana. Int. J. Mol. Sci. 2013, 14, 14872-14891.

AMA Style

Zhang X-L, Yang Z-P, Zhang J, Zhang L-G. Ectopic Expression of BraYAB1-702, a Member of YABBY Gene Family in Chinese Cabbage, Causes Leaf Curling, Inhibition of Development of Shoot Apical Meristem and Flowering Stage Delaying in Arabidopsis thaliana. International Journal of Molecular Sciences. 2013; 14(7):14872-14891.

Chicago/Turabian Style

Zhang, Xin-Ling; Yang, Ze-Ping; Zhang, Jing; Zhang, Lu-Gang. 2013. "Ectopic Expression of BraYAB1-702, a Member of YABBY Gene Family in Chinese Cabbage, Causes Leaf Curling, Inhibition of Development of Shoot Apical Meristem and Flowering Stage Delaying in Arabidopsis thaliana." Int. J. Mol. Sci. 14, no. 7: 14872-14891.


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