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Int. J. Mol. Sci. 2013, 14(7), 14270-14286; doi:10.3390/ijms140714270
Article

Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays

1
, 2
, 3,* , 4
, 4,*  and 1,2,5,*
Received: 20 May 2013; in revised form: 18 June 2013 / Accepted: 28 June 2013 / Published: 9 July 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs)
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Abstract: Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs are unknown. Using customized miRNA array analysis, we identified miRNAs from A. thaliana ecotype Columbia (Col-0) regulated by 24-epibrassinolide (EBR, a highly active BR). We found that miR395a was significantly up-regulated by EBR treatment and validated its expression under these conditions. miR395a was over expressed in leaf veins and root tissues in EBR-treated miR395a promoter::GUS plants. We integrated bioinformatics methods and publicly available DNA microarray data to predict potential targets of miR395a. GUN5—a multifunctional protein involved in plant metabolic functions such as chlorophyll synthesis and the abscisic acid (ABA) pathway—was identified as a possible target. ABI4 and ABI5, both genes positively regulated by ABA, were down-regulated by EBR treatment. In summary, our results suggest that EBR regulates seedling development and root growth of A. thaliana through miR395a by suppressing GUN5 expression and its downstream signal transduction.
Keywords: brassinosteroids; miR395a; root growth; Arabidopsis thaliana; microRNA array brassinosteroids; miR395a; root growth; Arabidopsis thaliana; microRNA array
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lin, L.-L.; Wu, C.-C.; Huang, H.-C.; Chen, H.-J.; Hsieh, H.-L.; Juan, H.-F. Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays. Int. J. Mol. Sci. 2013, 14, 14270-14286.

AMA Style

Lin L-L, Wu C-C, Huang H-C, Chen H-J, Hsieh H-L, Juan H-F. Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays. International Journal of Molecular Sciences. 2013; 14(7):14270-14286.

Chicago/Turabian Style

Lin, Li-Ling; Wu, Chia-Chi; Huang, Hsuan-Cheng; Chen, Huai-Ju; Hsieh, Hsu-Liang; Juan, Hsueh-Fen. 2013. "Identification of MicroRNA 395a in 24-Epibrassinolide-Regulated Root Growth of Arabidopsis thaliana Using MicroRNA Arrays." Int. J. Mol. Sci. 14, no. 7: 14270-14286.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert