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The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms
Department of Pharmacy, University "G. d'Annunzio", Chieti-Pescara, via dei Vestini 31, 66100 Chieti, Italy
Medicine, Surgery and Health Sciences Department, University of Trieste, Piazza dell'Ospitale 1, 34129 Trieste, Italy
Department of Life Sciences, University of Trieste, via Giorgieri 5, 34127 Trieste, Italy
* Author to whom correspondence should be addressed.
Received: 11 April 2013; in revised form: 5 June 2013 / Accepted: 6 June 2013 / Published: 28 June 2013
Abstract: In this work, we studied the antimicrobial properties of a nanocomposite system based on a lactose-substituted chitosan and silver nanoparticles: Chitlac-nAg. Twofold serial dilutions of the colloidal Chitlac-nAg solution were both tested on Streptococcus mitis, Streptococcus mutans, and Streptococcus oralis planktonic phase and biofilm growth mode as well as on saliva samples. The minimum inhibitory and bactericidal concentrations of Chitlac-nAg were evaluated together with its effect on sessile cell viability, as well as both on biofilm formation and on preformed biofilm. In respect to the planktonic bacteria, Chitlac-nAg showed an inhibitory/bactericidal effect against all streptococcal strains at 0.1% (v/v), except for S. mitis ATCC 6249 that was inhibited at one step less. On preformed biofilm, Chitlac-nAg at a value of 0.2%, was able to inhibit the bacterial growth on the supernatant phase as well as on the mature biofilm. For S. mitis ATCC 6249, the biofilm inhibitory concentration of Chitlac-nAg was 0.1%. At sub-inhibitory concentrations, the Streptococcal strains adhesion capability on a polystyrene surface showed a general reduction following a concentration-dependent-way; a similar effect was obtained for the metabolic biofilm activity. From these results, Chitlac-nAg seems to be a promising antibacterial and antibiofilm agent able to hinder plaque formation.
Keywords: silver-nanoparticles; polysaccharide; antimicrobial activity; oral Streptococci; planktonic and sessile growth; saliva
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Di Giulio, M.; Di Bartolomeo, S.; Di Campli, E.; Sancilio, S.; Marsich, E.; Travan, A.; Cataldi, A.; Cellini, L. The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms. Int. J. Mol. Sci. 2013, 14, 13615-13625.
Di Giulio M, Di Bartolomeo S, Di Campli E, Sancilio S, Marsich E, Travan A, Cataldi A, Cellini L. The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms. International Journal of Molecular Sciences. 2013; 14(7):13615-13625.
Di Giulio, Mara; Di Bartolomeo, Soraya; Di Campli, Emanuela; Sancilio, Silvia; Marsich, Eleonora; Travan, Andrea; Cataldi, Amelia; Cellini, Luigina. 2013. "The Effect of a Silver Nanoparticle Polysaccharide System on Streptococcal and Saliva-Derived Biofilms." Int. J. Mol. Sci. 14, no. 7: 13615-13625.