- freely available
Int. J. Mol. Sci. 2013, 14(7), 13447-13462; doi:10.3390/ijms140713447
Published: 27 June 2013
Abstract: Arginine-Glycine-Aspartic (RGD), is the specific recognition site of integrins with theirs ligands, and regulates cell-cell and cell-extracellular matrix interactions. The RGD motif can be combined with integrins overexpressed on the tumor neovasculature and tumor cells with a certain affinity, becoming the new target for imaging agents, and drugs, and gene delivery for tumor treatment. Further, RGD as a biomimetic peptide can also promote cell adherence to the matrix, prevent cell apoptosis and accelerate new tissue regeneration. Functionalizing material surfaces with RGD can improve cell/biomaterial interactions, which facilitates the generation of tissue-engineered constructs. This paper reviews the main functions and advantages of RGD, describes the applications of RGD in imaging agents, drugs, gene delivery for tumor therapy, and highlights the role of RGD in promoting the development of tissue engineering (bone regeneration, cornea repair, artificial neovascularization) in recent years.
Arginine-glycine-aspartic (RGD) (Figure 1), is a cell adhesion motif displayed on many extracellular matrix (ECM) and plasma proteins . Since RGD was first identified as specific binding sites for fibronectin (FN) and the FN receptor , it has attracted widespread attention and research. Many glycoproteins such as laminin, vitronectin (VN), fibrinogen (Fg), von Willebrand factor (vWF), osteopontin, etc. , have been found in the ECM, and they are RGD-adhesive proteins. RGD plays an important role in cell recognition and cell adhesion, it has been used into tumor therapy and tissue engineering by recombinant means and some chemical methods.
Hynes  had reported that the membrane proteins associated with ECM glycoprotein receptors on the cell surface were called integrins, which were members of the adhesion receptors. The binding of integrins to theirs ligands were dependent on divalent cations to mediate cell-cell and cell-matrix adhesion. Thus, integrins constituted cell adhesion receptors not only for cell-matrix adhesion but also for signaling bidirectionally across the membrane. The large heterodimeric cell surface receptors-integrins were found in many animal species ranging from sponges to mammals . They were involved in fundamental cellular processes such as attachment migration, proliferation, differentiation, and survival. Integrins also contributed to the initiation and progression of many biological diseases such as angiogenesis, thrombosis, inflammation, osteoporosis neoplasia, tumor metastasis and gene expression .
RGD-based ligands for integrins are studied in pathology and pharmacology. Furthermore, the RGD-integrin system is exploited to target cell recognition and internalization, which is applied to man-made constructs by mimicking the pathogens. This system enables the study of many aspects (such as diagnostics, therapeutics and the regenerating of transplanted tissue. RGD modified drugs and imaging agents have been investigated and developed by conjugation of the RGD-peptides with a carrier device. The carrier device has been equipped with drug molecules or reporter molecules. RGD-peptides and RGD-mimetics have also been applied to modify liposomes, polymers and peptides by chemical means to improve the biological effects of therapeutic agents. Additionally, RGD-peptides were utilized in gene delivery by viral and non-viral vectors . The surface modification technology with fixed RGD peptides has promoted the application of integrin-mediated cell adhesion to develop tissue engineering, especially for biomaterials.
2. The Functions of RGD
2.1. The Sequence and the Structure of RGD
The sequence and the structure of RGD-containing peptides have linear and cyclic RGD peptides. However, the cyclic RGD peptides display a higher activity compared to the linear RGD peptides. The advantages of cyclic peptides may be due to a conformationally less flexible structure to resist proteolysis and have the ability to bind with higher affinities to integrin receptors [8,9]. Enwerem et al.  searched 10 models of cyclic-five-member-ring pentapeptides containing Arginine-Glycine-Aspartic acid (RGD). Among the investigated models, cRGDfV, cRGDfE, cRGDfC, cRGDyV, and cRGDfK represented active RGD peptides. The other five models (cRGDVf, cRGDEf, cRGDCf, cRGDVy, and cRGDKf) were modified (five structures) forms of the previous mentioned RGD species. Among the modified models, cRGDEf and cRGDCf were expected as the new active candidates for the active cyclic RGD pentapeptides.
RGD peptides for scientific research and practical applications have some advantages [11,12]: (i) RGD is much smaller as compared to monoclonal antibodies, and RGD conjugates can have easier access to the tumor tissue; (ii) the use of RGD minimizes the risk of immune reactivity or pathogen transfer; (iii) the synthesis of RGD peptides is relatively simple and inexpensive, which facilitates translation into the clinic; (iv) the applications of RGD are much wider than folic acid. Not only is RGD used in tumor therapy, but it can also be coupled to material surfaces in controlled densities and orientations.
2.2. RGD-Mediated Recognition and Adhesion with Cells
RGD, is a cell recognition and attachment site for a number of extracellular matrix proteins as well as blood and cell surface proteins, and has important regulatory functions in many biological activities. RGD is involved in cell attachment, cell spreading, actin-skeleton formation, and focal-adhesion formation with integrins. These four overlapped reactions are important for transmitting signals related to cell behavior and the cell cycle .
Integrins α5β3 and αvβ3 are members of the heterodimeric glycoprotein receptors; they regulate cell-cell and cell-extracellular matrix interactions. Integrin αvβ3 plays a key role in the early stage of angiogenesis; it is expressed at low levels on mature endothelial cells and epithelial cells, but is highly expressed on the activated endothelial cells of tumor neovasculature and other tumor cells, including osteosarcomas, neuroblastomas, glioblastomas, melanomas, lung carcinomas, and breast cancer . The α5β3 and αvβ3 have strong sequence similarity around the RGD binding site, but α5β3 is recognized by RGD through “one-side” interaction while αvβ3 is through “side-on” interaction . The two interactions reveal subtle differences in the location of the conserved Asp in the β propeller groove of the respective α subunits as depicted in Figure 2. That is, in αv, the conserved Asp218 is at the entry of a shallow groove and located on the side while Asp150 is present on the opposite side of this pocket; Asp150 offers an extra H-bond interaction opportunity. Both Asp residues provide a particularly strong interaction by interacting with the arginine. A Thr212 occupies the bottom of the groove. However, in α5, Asp227 occupies the same location at the entry of the pocket as Asp218 (αv), but Asp150 is changed for a small non polar Ala159 and Thr212 in the bottom is replaced by a larger Gln221 in α5. Integrin αIIbβ3 as αll osteric receptor for fibrinogen, only the activated and high-affinity αIIbβ3 interacts with soluble fibrinogen by being dependent on the RGD . In addition, RGD also recognizes α3β1, α8β1, αvβ1, αvβ6, and αvβ5 of the integrin family . The RGD recognition motif makes two key and conserved interactions with both α and β subunits to mediate ligand binding. The guanidine function of arginine is engaged in a bidentate salt bridge with a highly conserved aspartic acid residue in the α subunit of the receptor. The carboxylate group of the aspartic acid coordinates with the metal-ion dependent adhesion site; the adhesion site is located in β subunit of the receptor .
In tissue engineering, efficient immobilization of the RGD peptide on the biomaterial surface is important. Cells make contact with cell surface receptors with adhesion in the ECM. It has been reported that the RGD peptide increased cell adhesion and had been used as an artificial ECM protein to induce specific cellular responses and to promote new tissue formation .
3. The Applications of RGD
Because integrins participate in cell-cell adhesion, cellular differentiation, migration, and attachment to the ECM, RGD peptides are extensively used in many diverse physiological and pathological processes, which mainly focus on the diagnosis and treatment of tumors, the development of anti-cancer drugs and antithrombotic drugs, and tissue engineering (bone regeneration, cornea repair, and artificial neovascularization, etc.).
3.1. Radiolabelled RGD Peptides for Tumor Imaging and Diagnostics
Tumor imaging has high specificity and sensitivity through conjugation of the RGD-peptide to imaging agents. This important technology has been widely used for the early diagnosis and differential diagnosis of tumors, and in clinical analysis and treatment.
RGD has a relatively high and specific affinity for αvβ3 integrins over-expressed in tumour neovasculature. An integrin αvβ3-targeted radiotracer design was simply visualized (; see Figure 3). The RGD peptide (targeting biomolecule) served as a vehicle to carry the radionuclide to the integrin αvβ3 overexpressed on tumor cells. Some progress in tumor-targeted imaging was made by single photon emission computed tomography (SPECT), positron emission tomography (PET), near-infrared fluorescence (NIRF), molecular magnetic resonance imaging (MRI) or photoacoustic imaging with the aid of RGD . Choi et al.  have synthesized a fusion protein, cyclic arginine-glycine-aspartate (RGD)-HSA-TIMP2. This protein composite was labeled with 123I- and 68Ga and was evaluated for in vivo tumor imaging using SPECT and PET. In SPECT and SPECT/CT images of 123I-HSA-TIMP2 (Figure 4(A), left) and 123I-RGD-HSA-TIMP2 (Figure 4(A), right) for 4 h after injection, it was observed that 123IHSA-TIMP2 was not absorbed by the mice bearing U87MG xenografts, but the level of tumor uptake of 123I-HSA-RGD-TIMP2 was slightly higher. In PET and PET/CT images of 68Ga-NOTA-HSA-TIMP2 (Figure 4(B), left) and 68Ga-NOTA-RGD-HSA-TIMP2 (Figure 4(B), right) for 3 h after injection, the level of tumor uptake of 68Ga-NOTA-RGD-HSA-TIMP2 was slightly higher than that of 68Ga-NOTA-HSA-TIMP2, when no tumor uptake of 68Ga-NOTA-HSA-TIMP2 was observed (Figure 4). These results demonstrated that the new fusion protein had potential not only as an anticancer agent but also as a radioligand for the diagnosis of tumors. Zerda et al.  showed a contrast agent for photoacoustic imaging of tumors; this contrast agent consisted of single-walled carbon nanotubes conjugated with cyclic RGD peptides. The results showed that these targeted nanotubes to mice bearing tumours displayed eight times greater photoacoustic signal in the tumour than mice injected with non-targeted nanotubes. Li et al.  reported a class of safe and effective RGD-conjugated dendrimer-modified gold nanorods (RGD-dGNRs). These nanoprobes were verified to have great potential in tumor targeting, imaging, and selective photothermal therapy. The possible therapeutic mechanisms were summarized based on the large number of experiments (Figure 5). RGD-dGNR nanoprobes that were distributed into the whole body of mice with tumors (mainly entered into liver and tumor vessels), could target and bind with the αvβ3 integrins on the surface of melanoma A735 cells and inner walls of tumor vessels. RGD-dGNR damaged vascular endothelial cells and blocked the blood flow in tumor vessels by absorbing NIR laser and transferring laser energy into heat under NIR laser irradiation, which could result in the necrosis or disappearance of tumor tissues via inhibiting the newborn vessels in tumor tissues, and blocking supply chains of nutrients and oxygen to starve tumor cells. Park et al.  synthesized Gd-DOTA-RGD, as a potential tumor-target for MRI. This complex exhibited higher R1 relaxivity and moderately good specificity for the αvβ3 receptor in hepatocellular carcinoma in Hras12V transgenic mice. Wu et al.  showed that near infrared (NIR) fluorescent dyes conjugated cyclic RGD peptides could be used for noninvasive NIRF imaging to detect and semi-quantify tumor integrin expression.
Now, the method using radiolabelled RGD peptides for imaging has been translated from the laboratory to the clinic, and the clinical data is critical for the ultimate clinical value of imaging of integrin expression. Thus, large-scale trials using radiolabelled RGD peptides within the context of response assessment or evaluation of patient prognosis are warranted to define the ultimate role of imaging of integrin expression in the clinic.
3.2. RGD Inhibiting Tumor
3.2.1. RGD Peptides Affecting Tumor Cell Adhesion and Migration
Angiogenesis-associated integrin αvβ3 represents an attractive target for therapeutic intervention and plays a key role in tumor-induced angiogenesis and tumor growth because of highly up-regulated angiogenic endothelium cells. Kang, et al.  investigated that RGD-modified salmosin (with salmosin extracted from snake venom), markedly inhibited B16F10 melanoma cell adhesions to the extracellular matrix proteins and hampered B16F10 melanoma cell invasion through a matrigel-coated filter. The inhibition by salmosin was caused by blocking integrins expressed on the surface of B16F10 melanoma cells. This confirmed that RGD-modified salmosin could significantly inhibit solid tumor growth. The immunomodulators, phorbol 12-myristate 13-acetate (PMA) was able to inhibit SNB-19 and T98G cell attachment to fibronectin and vitronectint only by the addition of the tripeptide RGD . Mitjans, et al.  characterized the therapeutic properties of RGD peptides and monoclonal antibody (MAb) in vivo that acted as αv antagonists for blocking human melanoma tumor growth. The Cyclic RGD peptide targeted MAb could successfully inhibit αv integrin–mediated cellular adhesion and induce detachment of previously substrate-attached tumor cells.
3.2.2. RGD Peptides Inducing Tumor Cell Apoptosis
Anuradha et al.  reported that RGD triggered apoptosis at a concentration of 1 mmol/L by activating caspase-3, leading to DNA fragmentation and cell death; this showed that the RGD peptide could induce tumor apoptosis. Chen et al.  found that the synthetic RGD-tachyplesin inhibited the proliferation of TSU prostate cancer cells and B16 melanoma cells as well as endothelial cells in a dose-dependent manner in vitro and reduced tumor growth in vivo. In addition to RGD, the peptide sequence of RGDS, RGDF etc. could also induce apoptosis of tumor cells in some experiments [31,32].
3.2.3. RGD Peptides Inhibiting Tumor Angiogenesis
The αv-integrins (αvβ3, αvβ5) regulate the contact of activated endothelial cells to proteins of the extracellular matrix during tumour angiogenesis, which is a prerequisite for survival of endothelial cells. Eliceiri and Cheresh  investigated a methylated cyclic RGD-peptide that, as an αv-integrin antagonist, had a disastrous impact on angiogenesis, microcirculation, growth and metastasis formation of a solid tumor in vivo; the inhibition of αv-integrins by a cyclic RGD-peptide resulted in significant reduction of functional vessel density, retardation of tumour growth and metastasis in vivo. Chavakis et al.  confirmed that the RGD peptide could completely inhibit the pro-angiogenic role of early vascular endothelial growth factor (VEGF), and even a low dose had the necessary anti-degradation ability to achieve a therapeutic effect.
3.3. RGD-Modified Carriers
3.3.1. RGD-Modified Gene Carriers Treating Tumor
DNA complex-coated RGDs were prepared by covalent conjugation of the RGD-peptide to the various combinations of cationic polymers, lipids and peptides, after which the new RGD-modified compounds were applied to plasmid DNA and other short interfering RNA (siRNA). These carriers have been reported to be used for RGD-modified non-viral gene carriers (Table 1). Vachutinsky et al.  described the preparation of targetable polyplex micelles through ion complexation of the polymers (c(RGDfK)-PEG-P(Lys-SH)) with plasmid DNA (Figure 6). These RGD-modified cross-linked polyplex micelles carrying sFlt-1 plasmid DNA could inhibit tumor growth. The therapeutic activity of these micelles was achieved by their being accumulated in the tumor and interacting with endothelial cells and enhancing intracellular uptake through receptor-mediated endocytosis.
In addition, viruses, as efficient vectors, have also been used for introducing genetic material into mammalian cells. RGD-modified viruses for gene delivery were reported in Table 2, they could redirect interference genes to tumour cells or angiogenic blood vessels by introducing the additional RGD-peptides into adenoviruses.
3.3.2. RGD-Modified Drugs for Target Therapy
Since the discovery of the RGD motif as a potent ligand of cell surface integrins (mainly αvβ3), RGD-mediated small molecule and RGD-containing therapeutic peptides and proteins, have been used to control drug bio-distribution, and have become employed as agents for cell targeting and endosomal delivery . Because of integrin abundance in endothelial cells, and the vascularization of tumoral tissues, RGD-mediated drug delivery is of special interest in cancer therapies.
RGD-modified PEGylated polyamidoamine (PAMAM) dendrimer with doxorubicin (DOX) conjugated by acid-sensitive cis-aconityl linkage (RGD-PPCD), has been demonstrated to have a significantly prolonged half-life, and exhibited higher accumulation in brain tumor as compared to normal brain tissue. More importantly, this novel RGD-mediated drug demonstrated increased tumor targeting by binding with the integrin receptors overexpressed on tumor cells, and controlled release of free DOX in weakly acidic lysosomes . Wang et al.  had also synthesized a novel dextran-oleate-cRGDfK conjugated for the self-assembly of this nanodrug; in vitro assays proved that this nanodrug could directly and selectively target PTX to MDA-MB-231 cells for effective cell internalization, displaying anti-tumor characteristics superior to Taxol formulation. Therefore, the new DO-cRGDfK conjugate nanodrug could be utilized for effective drug or gene delivery nanoparticles to curb malignant metastatic cancer cells. The more various RGD-modified drugs constructs and descriptions are summarized in Table 3.
3.4. RGD Used in Tissue Engineering
Tissue engineering is a new discipline that has been developed in recent years, in which biological substitutes are used to repair, maintain or improve the function of biological tissues through the application of biology and engineering principles. An important concept in tissue engineering is that implanted substrates should not only provide structural support for damaged tissues, but also integrate with these tissues and promote regeneration in the surrounding area. RGD effectively promotes the attachment of numerous cell types to a plethora of diverse materials .
Integrin receptors can recognize and interact with the RGD motif, and the functionality of RGD can be maintained after processing and sterilization steps. Modification with fixed RGD peptides on biomaterial surfaces promotes the application of integrin-mediated cell adhesion, and thus encourages the development of tissue engineering. Integrins can control cell adhesion to biomaterial surfaces by interacting with adhesive extracellular ligands, and the mechanisms of this process may be through adsorbing, engineering and depositing as follows: (i) cells adsorb adhesive extracellular ligands (RGD) on biomaterial surfaces with integrins mediating; (ii) biomaterial is engineered at the interface by bioadhesive motifs (RGD) being adsorbed; (iii) more bioadhesive ligands are deposited by cells (Figure 7) .
Recently, Michal et al.  demonstrated that RGD-immobilized alginate scaffolds were beneficial to cardiac tissue engineering, because the immobilized RGD peptide promoted cell adherence to the matrix, prevented cell apoptosis, increased cell survival and recovery, and accelerated cardiac tissue regeneration. Other approaches for RGD-mediated modifying of biomaterials are listed in Table 4.
3.4.1. Bone Regeneration
RGD-based ligands of integrins act as anchor points, because the actin cytoskeleton can bind to these anchor points and form structural components of the cell. The extracellular adhesive proteins, especially the RGD, promote cell adhesion and migration, which play particularly important roles in the osseointegration behavior of osteoblasts. The improved osseointegration observed on RGD-coated surfaces has stimulated recent efforts to attach RGD to oxide coated metals and their alloys such as Ti and Ti-6Al-4V. Chen et al.  had studied the combined effects of laser micro grooving and RGD-coating on the osseointegration of Ti-6Al-4V pins in vivo. The results demonstrated that immobilization of RGD peptides on titanium (Ti) surfaces enhanced implant bone healing by promoting early osteoblastic cell attachment and subsequent differentiation by facilitating integrin binding.
3.4.2. Cornea Repair
Cornea integrity is very critical for our eyes. Corneas may be damaged when cornea endothelial cells become less abundant with age or disease . Developments in tissue engineering have the potential to repair corneas. RGD-mediated surface modification facilitated cell attachment, proliferation, alignment and expression of both collagens (type I and V) and proteoglycans (decorin and biglycan). Gil et al.  had demonstrated that the RGD-coupled silk human cornea structure could be bio-functionalized by integrating corneal stroma tissue with proteoglycan-rich extracellular matrix. This biomimetic approach to replicate the structural hierarchy and architecture of corneal stromal tissue may become a useful strategy for engineering human cornea. Further, this approach can be exploited for other tissue systems to be repaired.
3.4.3. Artificial Neovascularization
The demand for small-diameter vascular grafts for cardiovascular disease is growing, so it is necessary to develop substitutes with bio-functionalities. RGD-modified grafts exhibited an improved inhibition of platelet adhesion, which enhanced cell infiltration, endothelium formation, smooth muscle regeneration and patency, the as-prepared PCL-RGD graft may be a promising candidate for the small-diameter vascular grafts .
In summary, the RGD-peptide sequence, as the specific recognition site of interaction between integrins and their ligands, has strong potential in applications for cancer therapy and tissue engineering. However, the functional mechanisms of RGD in cancer treatment and tissue engineering should be further investigated in order to close the gap between the experimental data and their translation to clinical application.
This work was financially supported by the National Natural Science Foundation of China (21076053, 20906016), Technological Research and Development Project of Hangzhou (20120232B13), China Postdoctoral Science Foundation funded project (2012M521198), Natural Science Foundation of Zhejiang Province (LY13B060008), and the Program for Changjiang Scholars and Innovative Research Team in Chinese University (IRT1231).
Conflict of Interest
The authors declare no conflict of interest.
- Colombo, M.; Bianchi, A. Click chemistry for the synthesis of rgd-containing integrin ligands. Molecules 2010, 15, 178–197. [Google Scholar]
- Pierschbacher, M.D.; Ruoslahti, E. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 1984, 309, 30–33. [Google Scholar]
- Kim, J.; Nam, H.Y.; Kim, T.-I.; Kim, P.-H.; Ryu, J.; Yun, C.-O.; Kim, S.W. Active targeting of RGD-conjugated bioreducible polymer for delivery of oncolytic adenovirus expressing shRNA against IL-8 mRNA. Biomaterials 2011, 32, 5158–5166. [Google Scholar]
- Hynes, R.O. Integrins: A family of cell surface receptors. Cell 1987, 48, 549–554. [Google Scholar]
- Yamada, K.M.; Geiger, B. Molecular interactions in cell adhesion complexes. Curr. Opin. Cell Biol 1997, 9, 76–85. [Google Scholar]
- Humphries, J.D.; Byron, A.; Humphries, M.J. Integrin ligands at a glance. J. Cell Sci 2006, 119, 3901–3903. [Google Scholar]
- Temming, K.; Schiffelers, R.M.; Molema, G.; Kok, R.J. RGD-based strategies for selective delivery of therapeutics and imaging agents to the tumour vasculature. Drug Resist. Update 2005, 8, 381–402. [Google Scholar]
- Verrier, S.; Pallu, S.; Bareille, R.; Jonczyk, A.; Meyer, J.; Dard, M.; Amedee, J. Function of linear and cyclic RGD-containing peptides in osteoprogenitor cells adhesion process. Biomaterials 2002, 23, 585–596. [Google Scholar]
- Frochot, C.; Stasio, B.D.; Vanderesse, R.; Belgy, M.-J.; Dodeller, M.; Guillemin, F.; Viriot, M.-L.; Barberi-Heyob, M. Interest of RGD-containing linear or cyclic peptide targeted tetraphenylchlorin as novel photosensitizers for selective photodynamic activity. Bioorg. Chem 2007, 35, 205–220. [Google Scholar]
- Enwerem, I.; Wang, J.; Leszczynski, J. In search of active RGD peptides: Theoretical study of hydrogen bonding in five-member ring Cyclic-RGD isomers. Comput. Theor. Chem 2012, 998, 141–147. [Google Scholar]
- Hilgenbrink, A.R.; Low, P.S. Folate receptor-mediated drug targeting: From therapeutics to diagnostics. J. Pharm. Sci 2005, 94, 2135–2146. [Google Scholar]
- Bellis, S.L. Advantages of RGD peptides for directing cell association with biomaterials. Biomaterials 2011, 32, 4205–4210. [Google Scholar]
- Hwang, D.S.; Sim, S.B.; Cha, H.J. Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide. Biomaterials 2007, 28, 4039–4046. [Google Scholar]
- Zitzmann, S.; Ehemann, V.; Schwab, M. Arginine-glycine-aspartic acid (RGD)-peptide binds to both tumor and tumor-endothelial cells in vivo. Cancer Res 2002, 62, 5139–5143. [Google Scholar]
- Delouvrié, B.; Al-Kadhimi, K.; Arnould, J.-C.; Barry, S.T.; Cross, D.A.; Didelot, M.; Gavine, P.R.; Germain, H.; Harris, C.S.; Hughes, A.M. Structure activity relationship of a series of non peptidic RGD integrin antagonists targeting α5β1. Part 2. Bioorg. Med. Chem. Lett 2012, 22, 4117–4121. [Google Scholar]
- Bella, J.; Humphries, M.J. Cα–H···O=C hydrogen bonds contribute to the specificity of RGD cell-adhesion interactions. BMC Struct. Biol 2005, 5, 4, , doi:10.1186/1472-6807-5-4.. [Google Scholar]
- Plow, E.F.; Haas, T.A.; Zhang, L.; Loftus, J.; Smith, J.W. Ligand binding to integrins. J. Biol. Chem 2000, 275, 21785–21788. [Google Scholar]
- Delouvrié, B.; Al-Kadhimi, K.; Arnould, J.-C.; Barry, S.T.; Cross, D.A.; Didelot, M.; Gavine, P.R.; Germain, H.; Harris, C.S.; Hughes, A.M. Structure activity relationship of a series of non peptidic RGD integrin antagonists targeting α5β1. Part 1. Bioorg. Med. Chem. Lett 2012, 22, 4111–4116. [Google Scholar]
- Liu, S. Radiolabeled multimeric cyclic RGD peptides as integrin αvβ3 targeted radiotracers for tumor imaging. Mol. Pharm 2006, 3, 472–487. [Google Scholar]
- Haubner, R.; Wester, H.J. Radiolabeled tracers for imaging of tumor angiogenesis and evaluation of anti-angiogenic therapies. Curr. Pharm. Design 2004, 10, 1439–1455. [Google Scholar]
- Choi, N.; Kim, S.-M.; Hong, K.S.; Cho, G.; Cho, J.-H.; Lee, C.; Ryu, E.K. The use of the fusion protein RGD-HSA-TIMP2 as a tumor targeting imaging probe for SPECT and PET. Biomaterials 2011, 32, 7151–7158. [Google Scholar]
- Zerda, A.D.L.; Liu, Z.; Bodapati, S.; Teed, R.; Vaithilingam, S.; Khuri-Yakub, B.T.; Chen, X.; Dai, H.; Gambhir, S.S. Ultrahigh sensitivity carbon nanotube agents for photoacoustic molecular imaging in living mice. Nano Lett 2010, 10, 2168–2172. [Google Scholar]
- Li, Z.; Huang, P.; Zhang, X.; Lin, J.; Yang, S.; Liu, B.; Gao, F.; Xi, P.; Ren, Q.; Cui, D. RGD-conjugated dendrimer-modified gold nanorods for in vivo tumor targeting and photothermal therapy. Mol. Pharm 2009, 7, 94–104. [Google Scholar]
- Park, J.; Lee, J.J.; Jung, J.C.; Yu, D.Y.; Oh, C.; Ha, S.; Kim, T.J.; Chang, Y. Gd-DOTA conjugate of RGD as a potential tumor-targeting mri contrast agent. Chem Bio Chem 2008, 9, 2811–2813. [Google Scholar]
- Wu, Y.; Cai, W.; Chen, X. Near-infrared fluorescence imaging of tumor integrin αvβ3 expression with Cy7-labeled RGD multimers. Mol. Imaging Biol 2006, 8, 226–236. [Google Scholar]
- Kang, I.C.; Kim, D.S.; Jang, Y.; Chung, K.H. Suppressive mechanism of salmosin, a novel disintegrin in B16 melanoma cell metastasis. Biochem. Bioph. Res. Comm 2000, 275, 169–173. [Google Scholar]
- Ritchie, C.K.; Giordano, A.; Khalili, K. Integrin involvement in glioblastoma multiforme: Possible regulation by NF-κB. J. Cell Physiol 2000, 184, 214–221. [Google Scholar]
- Mitjans, F.; Meyer, T.; Fittschen, C.; Goodman, S.; Jonczyk, A.; Marshall, J.F.; Reyes, G.; Piulats, J. In vivo therapy of malignant melanoma by means of antagonists of αv integrins. Int. J. Cancer 2000, 87, 716–723. [Google Scholar]
- Anuradha, C.; Kanno, S.; Hirano, S. RGD peptide-induced apoptosis in human leukemia HL-60 cells requires caspase-3 activation. Cell Biol. Toxicol 2000, 16, 275–283. [Google Scholar]
- Chen, Y.; Xu, X.; Hong, S.; Chen, J.; Liu, N.; Underhill, C.B.; Creswell, K.; Zhang, L. RGD-Tachyplesin inhibits tumor growth. Cancer Res 2001, 61, 2434–2438. [Google Scholar]
- Aguzzi, M.S.; Giampietri, C.; de Marchis, F.; Padula, F.; Gaeta, R.; Ragone, G.; Capogrossi, M.C.; Facchiano, A. RGDS peptide induces caspase 8 and caspase 9 activation in human endothelial cells. Blood 2004, 103, 4180–4187. [Google Scholar]
- Chen, X.; Wang, J.; Fu, B.; Yu, L. RGD-containing peptides trigger apoptosis in glomerular mesangial cells of adult human kidneys. Biochem. Bioph. Res. Comm 1997, 234, 594–599. [Google Scholar]
- Eliceiri, B.P.; Cheresh, D.A. The role of alphav integrins during angiogenesis: Insights into potential mechanisms of action and clinical development. J. Clin. Invest 1999, 103, 1227–1230. [Google Scholar]
- Chavakis, E.; Riecke, B.; Lin, J.; Linn, T.; Bretzel, R.; Preissner, K.; Brownlee, M.; Hammes, H.P. Kinetics of integrin expression in the mouse model of proliferative retinopathy and success of secondary intervention with cyclic RGD peptides. Diabetologia 2002, 45, 262–267. [Google Scholar]
- Vachutinsky, Y.; Oba, M.; Miyata, K.; Hiki, S.; Kano, M.R.; Nishiyama, N.; Koyama, H.; Miyazono, K.; Kataoka, K. Antiangiogenic gene therapy of experimental pancreatic tumor by sFlt-1 plasmid DNA carried by RGD-modified crosslinked polyplex micelles. J. Control. Release 2011, 149, 51–57. [Google Scholar]
- Yonenaga, N.; Kenjo, E.; Asai, T.; Tsuruta, A.; Shimizu, K.; Dewa, T.; Nango, M.; Oku, N. RGD-based active targeting of novel polycation liposomes bearing siRNA for cancer treatment. J. Control. Release 2012, 160, 177–181. [Google Scholar]
- Orsi, S.; Guarnieri, D.; de Capua, A.; Netti, P.A. Gene-activated and cell-migration guiding PEG matrices based on three dimensional patterning of RGD peptides and DNA complexes. Acta Biomater 2012, 8, 3228–3240. [Google Scholar]
- Zhan, C.; Meng, Q.; Li, Q.; Feng, L.; Zhu, J.; Lu, W. Cyclic RGD-polyethylene glycol-polyethylenimine for intracranial glioblastoma-targeted gene delivery. Chem. Asian J 2012, 7, 91–96. [Google Scholar]
- Sakae, M.; Ito, T.; Yoshihara, C.; Iida-Tanaka, N.; Yanagie, H.; Eriguchi, M.; Koyama, Y. Highly efficient in vivo gene transfection by plasmid/PEI complexes coated by anionic PEG derivatives bearing carboxyl groups and RGD peptide. Biomed. Pharmacother 2008, 62, 448–453. [Google Scholar]
- Hood, J.D.; Bednarski, M.; Frausto, R.; Guccione, S.; Reisfeld, R.A.; Xiang, R.; Cheresh, D.A. Tumor regression by targeted gene delivery to the neovasculature. Science 2002, 296, 2404–2407. [Google Scholar]
- Jiang, J.; Yang, S.; Wang, J.; Yang, L.; Xu, Z.; Yang, T.; Liu, X.; Zhang, Q. Sequential treatment of drug-resistant tumors with RGD-modified liposomes containing siRNA or doxorubicin. Eur. J. Pharm. Biopharm 2010, 76, 170–178. [Google Scholar]
- Kibria, G.; Hatakeyama, H.; Ohga, N.; Hida, K.; Harashima, H. Dual-ligand modification of PEGylated liposomes shows better cell selectivity and efficient gene delivery. J. Control. Release 2011, 153, 141–148. [Google Scholar]
- Samanta, S.; Sistla, R.; Chaudhuri, A. The use of RGDGWK-lipopeptide to selectively deliver genes to mouse tumor vasculature and its complexation with p53 to inhibit tumor growth. Biomaterials 2010, 31, 1787–1797. [Google Scholar]
- Leng, Q.; Mixson, A.J. Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection. Nucleic Acids Res. 2005, 33. [Google Scholar] [CrossRef]
- Przystal, J.M.; Umukoro, E.; Stoneham, C.A.; Yata, T.; O’Neill, K.; Syed, N.; Hajitou, A. Proteasome inhibition in cancer is associated with enhanced tumor targeting by the adeno-associated virus/phage. Mol. Oncol 2013, 7, 55–66. [Google Scholar]
- Katayama, K.; Furuki, R.; Yokoyama, H.; Kaneko, M.; Tachibana, M.; Yoshida, I.; Nagase, H.; Tanaka, K.; Sakurai, F.; Mizuguchi, H. Enhanced in vivo gene transfer into the placenta using RGD fiber-mutant adenovirus vector. Biomaterials 2011, 32, 4185–4193. [Google Scholar]
- Meyer, A.; Auernheimer, J.; Modlinger, A.; Kessler, H. Targeting RGD recognizing integrins: Drug development, biomaterial research, tumor imaging and targeting. Curr. Pharm. Design 2006, 12, 2723–2747. [Google Scholar]
- Zhu, S.; Qian, L.; Hong, M.; Zhang, L.; Pei, Y.; Jiang, Y. RGD-modified PEG-PAMAM-DOX conjugate: In vitro and in vivo targeting to both tumor neovascular endothelial cells and tumor cells. Adv. Mater 2011, 23, H84–H89. [Google Scholar]
- Wang, Z.; Lee, T.Y.; Ho, P.C. A novel dextran-oleate-cRGDfK conjugate for self-assembly of nanodrug. Nanomed. Nanotechnol 2012, 8, 194–203. [Google Scholar]
- Xu, Q.; Liu, Y.; Su, S.; Li, W.; Chen, C.; Wu, Y. Anti-tumor activity of paclitaxel through dual-targeting carrier of cyclic RGD and transferrin conjugated hyperbranched copolymer nanoparticles. Biomaterials 2012, 33, 1627–1639. [Google Scholar]
- Zhang, L.; Zhu, S.; Qian, L.; Pei, Y.; Qiu, Y.; Jiang, Y. RGD-modified PEG-PAMAM-DOX conjugates: In vitro and in vivo studies for glioma. Eur. J. Pharm. Biopharm 2011, 79, 232–240. [Google Scholar]
- Zhang, Y.; Wang, J.; Bian, D.; Zhang, X.; Zhang, Q. Targeted delivery of RGD-modified liposomes encapsulating both combretastatin A-4 and doxorubicin for tumor therapy: In vitro and in vivo studies. Eur. J. Pharm. Biopharm 2010, 74, 467–473. [Google Scholar]
- Naik, S.; Patel, D.; Chuttani, K.; Mishra, A.K.; Misra, A. In vitro mechanistic study of cell death and in vivo performance evaluation of RGD grafted PEGylated docetaxel liposomes in breast cancer. Nanomed. Nanotechnol 2012, 8, 951–962. [Google Scholar]
- Yue, W.; Sun, Q.; Landreneau, R.; Wu, C.; Siegfried, J.M.; Yu, J.; Zhang, L. Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression. Cancer Res 2009, 69, 6339–6346. [Google Scholar]
- Yan, J.H.; Yang, G.W.; Wang, J.P.; Wu, N.; Zhuang, G.H. Gene expression and activity analysis of a novel fusion protein (RGD) 3/tTF. Chin. J. Biotechnol 2007, 23, 409–413. [Google Scholar]
- Tugulu, S.; Silacci, P.; Stergiopulos, N.; Klok, H.A. RGD—Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells. Biomaterials 2007, 28, 2536–2546. [Google Scholar]
- Tanabe, N.; Wheal, B.D.; Kwon, J.; Chen, H.H.; Shugg, R.; Sims, S.M.; Goldberg, H.A.; Dixon, S.J. Osteopontin signals through calcium and nuclear factor of activated T cells (NFAT) in osteoclasts: A novel RGD-dependent pathway promoting cell survival. J. Biol. Chem 2011, 286, 39871–39881. [Google Scholar]
- García, A.J. Get a grip: Integrins in cell-biomaterial interactions. Biomaterials 2005, 26, 7525–7529. [Google Scholar]
- Shachar, M.; Tsur-Gang, O.; Dvir, T.; Leor, J.; Cohen, S. The effect of immobilized RGD peptide in alginate scaffolds on cardiac tissue engineering. Acta Biomater 2011, 7, 152–162. [Google Scholar]
- Zheng, W.; Wang, Z.; Song, L.; Zhao, Q.; Zhang, J.; Li, D.; Wang, S.; Han, J.; Zheng, X.-L.; Yang, Z. Endothelialization and patency of RGD-functionalized vascular grafts in a rabbit carotid artery model. Biomaterials 2012, 33, 2880–2891. [Google Scholar]
- Zhang, H.; Lin, C.Y.; Hollister, S.J. The interaction between bone marrow stromal cells and RGD-modified three-dimensional porous polycaprolactone scaffolds. Biomaterials 2009, 30, 4063–4069. [Google Scholar]
- Tocce, E.; Liliensiek, S.; Broderick, A.; Jiang, Y.; Murphy, K.; Murphy, C.; Lynn, D.; Nealey, P. The influence of biomimetic topographic features and the extracellular matrix peptide RGD on human corneal epithelial contact guidance. Acta Biomater 2013, 9, 5040–5051. [Google Scholar]
- Hennessy, K.M.; Clem, W.C.; Phipps, M.C.; Sawyer, A.A.; Shaikh, F.M.; Bellis, S.L. The effect of RGD peptides on osseointegration of hydroxyapatite biomaterials. Biomaterials 2008, 29, 3075–3083. [Google Scholar]
- Chen, J.; Bly, R.; Saad, M.; AlKhodary, M.; El-Backly, R.; Cohen, D.; Kattamis, N.; Fatta, M.; Moore, W.; Arnold, C. In vivo study of adhesion and bone growth around implanted laser groove/RGD-functionalized Ti-6Al-4V pins in rabbit femurs. Mat. Sci. Eng. C 2011, 31, 826–832. [Google Scholar]
- Wohlrab, S.; Müller, S.; Schmidt, A.; Neubauer, S.; Kessler, H.; Leal-Egaña, A.; Scheibel, T. Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins. Biomaterials 2012, 33, 6650–6659. [Google Scholar]
- Patra, C.; Ricciardi, F.; Engel, F.B. The functional properties of nephronectin: An adhesion molecule for cardiac tissue engineering. Biomaterials 2012, 33, 4327–4335. [Google Scholar]
- Jo, D.H.; Lee, T.G.; Kim, J.H. Nanotechnology and nanotoxicology in retinopathy. Int. J. Mol. Sci 2011, 12, 8288–8301. [Google Scholar]
- Gil, E.S.; Mandal, B.B.; Park, S.-H.; Marchant, J.K.; Omenetto, F.G.; Kaplan, D.L. Helicoidal multi-lamellar features of RGD-functionalized silk biomaterials for corneal tissue engineering. Biomaterials 2010, 31, 8953–8963. [Google Scholar]
|Polymer||RGD-PEG-PCL||siRNA||B16F10-luc2 lung metastatic |
|RGD-PEG||PEG-PEI/DNA complexes||NIH3T3 cells |
|RGD-PEG-PEI||Plasmid DNA||Intracranial glioblastoma |
|RGD-PEG-Suc||Plasmid DNA coding luciferase||Melanoma cell |
|Lipid||Polymerized lipid nanoparticle||ATP-Raf||M21-L/CT26 colon carcinoma |
|Liposomes||RGD-SSL-DOX liposomes||RGD-Lipo-siRNA(MDR1)||Breast cancer MCF7/A cells |
|RGD-PEGylated liposomes||siRNA||Pigment epithelial cells |
|Peptides||RGDGWK-lipopeptde||Anti-cancer p53 gene||B16F10 tumor |
|RGD-HK-branched peptides||siLacZ, siLuciferase||MDA-MB-435c, MCF7 |
|Table 2. RGD-modified viruses for gene delivery.|
|RGD4C/AAV/phage hybrid||GFP or Luc reporter genes||Human M21 Melanoma Human U87 glioblastoma |
|Ad/CD-PEG500-RGD||shRNA||Various cancer cells |
|(Adenovirus) Ad-RGD||Firefly luciferase gene||Mouse TS cells |
|Table 3. RGD modified drugs.|
|small molecule drugs||Paclitaxel||PTX-RGD/Tf-NPs (nanoparticles)||HeLa cells |
|Doxorubicin||RGD-PEG-PAMAM-DOX||C6 glioma cells |
|Combretastatin A-4 (CA-4) and doxorubicin (Dox)||RGD-CA-4 and Dox liposomes||B16 and B16F10 melanoma cells |
|Docetaxel||RGD-PEG-LP-DC (Liposomes)||BT-20 and MDA-MB-231 cells |
|therapeutic proteins and peptides||Fibulin-5||RGD-fibulin-5||A549, H1299 and H460 cells |
|tTf||(RGD)3/tTF||H460 lung cancer cells |
|The angiogenic factor Del1||RGD-Mediated angiogenic factor||Human umbilical vein endothelial cells |
|Osteopontin||RGD-containing Osteopontin||Avian osteoclast-like cells |
|Table 4. RGD-peptide modified biomaterials.|
|Polymer||Alginate scaffolds||RGD-immobilized alginate scaffolds||Cardiac cell |
|PCL (polycaprolactone)||RGD-PCL||Vascular grafts rabbit carotid artery |
|PCL||RGD-modified 3D-PCL||Bone marrow stromal cells |
|Poly (ethylene imine)-poly(2-vinyl-4,4-dimethylazlactone)||RGD-PEI/PVDMA||Human corneal epithelial cell |
|Inorganic materials||Hydroxyapatite (HA)||RGD-coated HA||Rat tibiae |
|Rabbit femurs |
|Proteins||Spider silk||RGD-modified spider silk||BALB/3T3 mouse fibroblasts |
|Nephronectin||RGD nephronectin||Cardiomyocytes |
© 2013 by the authors; licensee MDPI, Basel, Switzerland This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).