Abstract: Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA is a frequent occurrence, its molecular mechanism remains unknown. A transcript-derived fragment (TDF72), which was obtained by cDNA amplified fragment length polymorphism (cDNA-AFLP), was up-regulated in the aborted buds and exhibited 89% sequence homology with the AtγVPE gene. In this study, TDF72 was used to clarify the role of VPE in FBA by isolation of the VPE gene RsVPE1 from radish flower buds. The full-length genomic DNA was 2346 bp including nine exons and eight introns. The full-length cDNA was 1825 bp, containing a complete open reading frame (ORF) of 1470 bp, which encoded a predicted protein containing 489 amino acid residues, with a calculated molecular mass of 53.735 kDa. Expression analysis demonstrated that RsVPE1 was expressed in all tested organs of radish at different levels. Highest expression was detected in aborted flower buds, suggesting that RsVPE1 has a role in FBA. In order to analyze the role of RsVPE1 in FBA, RsVPE1 was overexpressed in transgenic Arabidopsis thaliana plants. Aborted flower buds appeared in transgenic plants subjected to heat stress. In addition, RsVPE1 expression in the transgenic plants reached a maximum when subjected to heat stress for 24 h and increased by 2.1-fold to 2.8-fold in three homozygous transgenic lines. These results indicated that RsVPE1 led to FBA when its expression levels exceeded a particular threshold, and provided evidence for the involvement of RsVPE1 in promoting FBA under heat stress.
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Zhang, J.; Li, Q.-F.; Huang, W.-W.; Xu, X.-Y.; Zhang, X.-L.; Hui, M.-X.; Zhang, M.-K.; Zhang, L.-G. A Vacuolar Processing Enzyme RsVPE1 Gene of Radish Is Involved in Floral Bud Abortion under Heat Stress. Int. J. Mol. Sci. 2013, 14, 13346-13359.
Zhang J, Li Q-F, Huang W-W, Xu X-Y, Zhang X-L, Hui M-X, Zhang M-K, Zhang L-G. A Vacuolar Processing Enzyme RsVPE1 Gene of Radish Is Involved in Floral Bud Abortion under Heat Stress. International Journal of Molecular Sciences. 2013; 14(7):13346-13359.
Zhang, Jing; Li, Qing-Fei; Huang, Wei-Wei; Xu, Xiao-Yong; Zhang, Xin-Ling; Hui, Mai-Xia; Zhang, Ming-Ke; Zhang, Lu-Gang. 2013. "A Vacuolar Processing Enzyme RsVPE1 Gene of Radish Is Involved in Floral Bud Abortion under Heat Stress." Int. J. Mol. Sci. 14, no. 7: 13346-13359.