Abstract: Achillea millefolium L. is a member of the Asteraceae family and has been used in folk medicine in many countries. In this study, 19 compounds in A. millefolium essential oil (AM-EO) have been identified; the major components are artemisia ketone (14.92%), camphor (11.64%), linalyl acetate (11.51%) and 1,8-cineole (10.15%). AM-EO can suppress the inflammatory responses of lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophages, including decreased levels of cellular nitric oxide (NO) and superoxide anion production, lipid peroxidation and glutathione (GSH) concentration. This antioxidant activity is not a result of increased superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, but rather occurs as a result of the down-regulation of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and heme oxygenase-1 (HO-1) expression, thus reducing the inflammatory response. Therefore, AM-EO can be utilized in many applications, including the treatment of inflammatory diseases in the future.
Keywords: Achillea millefolium L.; antioxidant; anti-inflammatory; essential oil; gas chromatography-mass spectrometry (GC-MS); lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophages
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Chou, S.-T.; Peng, H.-Y.; Hsu, J.-C.; Lin, C.-C.; Shih, Y. Achillea millefolium L. Essential Oil Inhibits LPS-Induced Oxidative Stress and Nitric Oxide Production in RAW 264.7 Macrophages. Int. J. Mol. Sci. 2013, 14, 12978-12993.
Chou S-T, Peng H-Y, Hsu J-C, Lin C-C, Shih Y. Achillea millefolium L. Essential Oil Inhibits LPS-Induced Oxidative Stress and Nitric Oxide Production in RAW 264.7 Macrophages. International Journal of Molecular Sciences. 2013; 14(7):12978-12993.
Chou, Su-Tze; Peng, Hsin-Yi; Hsu, Jaw-Cherng; Lin, Chih-Chien; Shih, Ying. 2013. "Achillea millefolium L. Essential Oil Inhibits LPS-Induced Oxidative Stress and Nitric Oxide Production in RAW 264.7 Macrophages." Int. J. Mol. Sci. 14, no. 7: 12978-12993.