Abstract: An attempt was made to use functionalized graphene oxide (GO) to detect the Promyelocytic leukemia/Retinoic acid receptor α fusion gene (PML/RARα fusion gene), a marker gene of acute promyelocytic leukemia. The functionalized GO was prepared by chemical exfoliation method, followed by a polyethylene glycol grafting. It is found that the functionalized GO can selectively adsorb the fluorescein isothiocyanate (FITC)-labeled single-stranded DNA probe and quench its fluorescence. The probe can be displaced by the PML/RARα fusion gene to restore the fluorescence, which can be detected by laser confocal microscopy and flow cytometry. These can be used to detect the presence of the PML/RARα fusion gene. This detection method is verified to be fast, simple and reliable.
Keywords: PML/RARα fusion gene; graphene oxide; fluorescein isothiocyanate (FITC); laser confocal microscopy
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Li, R.; Tan, Y.; Chen, X.; Ren, F.; Zhang, Y.; Xu, Z.; Wang, H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. Int. J. Mol. Sci. 2013, 14, 12863-12872.
Li R, Tan Y, Chen X, Ren F, Zhang Y, Xu Z, Wang H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. International Journal of Molecular Sciences. 2013; 14(6):12863-12872.
Li, Ran; Tan, Yanhong; Chen, Xiuhua; Ren, Fanggang; Zhang, Yaofang; Xu, Zhifang; Wang, Hongwei. 2013. "Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide." Int. J. Mol. Sci. 14, no. 6: 12863-12872.