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Int. J. Mol. Sci. 2013, 14(6), 12863-12872; doi:10.3390/ijms140612863
Article

Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide

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Received: 25 March 2013 / Revised: 30 May 2013 / Accepted: 9 June 2013 / Published: 20 June 2013
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Abstract

An attempt was made to use functionalized graphene oxide (GO) to detect the Promyelocytic leukemia/Retinoic acid receptor α fusion gene (PML/RARα fusion gene), a marker gene of acute promyelocytic leukemia. The functionalized GO was prepared by chemical exfoliation method, followed by a polyethylene glycol grafting. It is found that the functionalized GO can selectively adsorb the fluorescein isothiocyanate (FITC)-labeled single-stranded DNA probe and quench its fluorescence. The probe can be displaced by the PML/RARα fusion gene to restore the fluorescence, which can be detected by laser confocal microscopy and flow cytometry. These can be used to detect the presence of the PML/RARα fusion gene. This detection method is verified to be fast, simple and reliable.
Keywords: PML/RARα fusion gene; graphene oxide; fluorescein isothiocyanate (FITC); laser confocal microscopy PML/RARα fusion gene; graphene oxide; fluorescein isothiocyanate (FITC); laser confocal microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Li, R.; Tan, Y.; Chen, X.; Ren, F.; Zhang, Y.; Xu, Z.; Wang, H. Detection of Promyelocytic Leukemia/Retinoic Acid Receptor α (PML/RARα) Fusion Gene with Functionalized Graphene Oxide. Int. J. Mol. Sci. 2013, 14, 12863-12872.

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