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Activity of Laccase Immobilized on TiO2-Montmorillonite Complexes
Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China
National Engineering Laboratory for Cereal Fermentation Technology & School of Biotechnology, Jiangnan University, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 11 February 2013; in revised form: 3 June 2013 / Accepted: 6 June 2013 / Published: 14 June 2013
Abstract: The TiO2-montmorillonite (TiO2-MMT) complex was prepared by blending TiO2 sol and MMT with certain ratio, and its properties as an enzyme immobilization support were investigated. The pristine MMT and TiO2-MMT calcined at 800 °C (TiO2-MMT800) were used for comparison to better understand the immobilization mechanism. The structures of the pristine MMT, TiO2-MMT, and TiO2-MMT800 were examined by HR-TEM, XRD and BET. SEM was employed to study different morphologies before and after laccase immobilization. Activity and kinetic parameters of the immobilized laccase were also determined. It was found that the TiO2 nanoparticles were successfully introduced into the MMT layer structure, and this intercalation enlarged the “d value” of two adjacent MMT layers and increased the surface area, while the calcination process led to a complete collapse of the MMT layers. SEM results showed that the clays were well coated with adsorbed enzymes. The study of laccase activity revealed that the optimum pH and temperature were pH = 3 and 60 °C, respectively. In addition, the storage stability for the immobilized laccase was satisfactory. The kinetic properties indicated that laccase immobilized on TiO2-MMT complexes had a good affinity to the substrate. It has been proved that TiO2-MMT complex is a good candidate for enzyme immobilization.
Keywords: montmorillonite; laccase; enzyme immobilization; TiO2
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MDPI and ACS Style
Wang, Q.; Peng, L.; Li, G.; Zhang, P.; Li, D.; Huang, F.; Wei, Q. Activity of Laccase Immobilized on TiO2-Montmorillonite Complexes. Int. J. Mol. Sci. 2013, 14, 12520-12532.
Wang Q, Peng L, Li G, Zhang P, Li D, Huang F, Wei Q. Activity of Laccase Immobilized on TiO2-Montmorillonite Complexes. International Journal of Molecular Sciences. 2013; 14(6):12520-12532.
Wang, Qingqing; Peng, Lin; Li, Guohui; Zhang, Ping; Li, Dawei; Huang, Fenglin; Wei, Qufu. 2013. "Activity of Laccase Immobilized on TiO2-Montmorillonite Complexes." Int. J. Mol. Sci. 14, no. 6: 12520-12532.