Int. J. Mol. Sci. 2013, 14(6), 12073-12089; doi:10.3390/ijms140612073
Article

Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase

1 State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China 2 School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China
* Author to whom correspondence should be addressed.
Received: 6 April 2013; in revised form: 19 May 2013 / Accepted: 31 May 2013 / Published: 6 June 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract: Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-grafted magnetic chitosan microspheres (HG-MCM) were prepared using reversed-phase suspension polymerization method. The HG-MCM presented a core-shell structure and regular spherical shape with poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) grafted onto the chitosan layer coating the Fe3O4 cores. The average diameter of the magnetic microspheres was 10.67 μm, within a narrow size distribution of 6.6–17.4 μm. The saturation magnetization and retentivity of the magnetic microspheres were 7.0033 emu/g and 0.6273 emu/g, respectively. The application of HG-MCM in immobilization of lactase showed that the immobilized enzyme presented higher storage, pH and thermal stability compared to the free enzyme. This indicates that HG-MCM have potential applications in bio-macromolecule immobilization.
Keywords: magnetic chitosan microspheres; reversed-phase suspension polymerization; immobilization; lactase

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MDPI and ACS Style

Zhao, W.; Yang, R.-J.; Qian, T.-T.; Hua, X.; Zhang, W.-B.; Katiyo, W. Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase. Int. J. Mol. Sci. 2013, 14, 12073-12089.

AMA Style

Zhao W, Yang R-J, Qian T-T, Hua X, Zhang W-B, Katiyo W. Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase. International Journal of Molecular Sciences. 2013; 14(6):12073-12089.

Chicago/Turabian Style

Zhao, Wei; Yang, Rui-Jin; Qian, Ting-Ting; Hua, Xiao; Zhang, Wen-Bin; Katiyo, Wendy. 2013. "Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase." Int. J. Mol. Sci. 14, no. 6: 12073-12089.

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