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Int. J. Mol. Sci. 2013, 14(6), 12073-12089; doi:10.3390/ijms140612073
Article

Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase

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Received: 6 April 2013; in revised form: 19 May 2013 / Accepted: 31 May 2013 / Published: 6 June 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract: Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-grafted magnetic chitosan microspheres (HG-MCM) were prepared using reversed-phase suspension polymerization method. The HG-MCM presented a core-shell structure and regular spherical shape with poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) grafted onto the chitosan layer coating the Fe3O4 cores. The average diameter of the magnetic microspheres was 10.67 μm, within a narrow size distribution of 6.6–17.4 μm. The saturation magnetization and retentivity of the magnetic microspheres were 7.0033 emu/g and 0.6273 emu/g, respectively. The application of HG-MCM in immobilization of lactase showed that the immobilized enzyme presented higher storage, pH and thermal stability compared to the free enzyme. This indicates that HG-MCM have potential applications in bio-macromolecule immobilization.
Keywords: magnetic chitosan microspheres; reversed-phase suspension polymerization; immobilization; lactase magnetic chitosan microspheres; reversed-phase suspension polymerization; immobilization; lactase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhao, W.; Yang, R.-J.; Qian, T.-T.; Hua, X.; Zhang, W.-B.; Katiyo, W. Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase. Int. J. Mol. Sci. 2013, 14, 12073-12089.

AMA Style

Zhao W, Yang R-J, Qian T-T, Hua X, Zhang W-B, Katiyo W. Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase. International Journal of Molecular Sciences. 2013; 14(6):12073-12089.

Chicago/Turabian Style

Zhao, Wei; Yang, Rui-Jin; Qian, Ting-Ting; Hua, Xiao; Zhang, Wen-Bin; Katiyo, Wendy. 2013. "Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase." Int. J. Mol. Sci. 14, no. 6: 12073-12089.


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