Int. J. Mol. Sci. 2013, 14(6), 11084-11095; doi:10.3390/ijms140611084
Article

Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice

Received: 9 April 2013; in revised form: 8 May 2013 / Accepted: 15 May 2013 / Published: 24 May 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Recently, senescence marker protein-30 (SMP30) knockout (KO) mice have been reported to be susceptible to apoptosis, however, the role of SMP30 has not been characterized in the small intestine. The aim of the present study is to investigate the role of SMP30 in the process of spontaneous and γ-radiation-induced apoptosis in mouse small intestine. Eight-week-old male wild-type (WT) mice and SMP30 KO mice were examined after exposure to 0, 1, 3, 5, and 9 Gy of γ-radiation. Apoptosis in the crypts of the small intestine increased in the 0 to 5 Gy radiated SMP30 KO and WT mice. Radiation-induced apoptosis and the BAX/Bcl-2 ratio in the SMP30 KO mice were significantly increased in comparison to each identically treated group of WT mice (p < 0.05). The levels of spontaneous apoptosis in both WT and KO mice were similar (p > 0.05), indicating that increased apoptosis of crypt cells of SMP30 KO by irradiation can be associated with SMP30 depletion. These results suggested that SMP30 might be involved in overriding the apoptotic homeostatic mechanism in response to DNA damage.
Keywords: SMP30; radiation; BAX; Bcl-2; intestine
PDF Full-text Download PDF Full-Text [870 KB, uploaded 19 June 2014 04:54 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Goo, M.-J.; Park, J.-K.; Hong, I.-H.; Kim, A.-Y.; Lee, E.-M.; Lee, E.-J.; Hwang, M.; Jeong, K.-S. Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice. Int. J. Mol. Sci. 2013, 14, 11084-11095.

AMA Style

Goo M-J, Park J-K, Hong I-H, Kim A-Y, Lee E-M, Lee E-J, Hwang M, Jeong K-S. Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice. International Journal of Molecular Sciences. 2013; 14(6):11084-11095.

Chicago/Turabian Style

Goo, Moon-Jung; Park, Jin-Kyu; Hong, Il-Hwa; Kim, Ah-Young; Lee, Eun-Mi; Lee, Eun-Joo; Hwang, Meeyul; Jeong, Kyu-Shik. 2013. "Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice." Int. J. Mol. Sci. 14, no. 6: 11084-11095.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert